Regional transendothelial Glycerol kinetics in humans

S. Klein, S. Coppack, D. Chinkes, J. Villanueva-Mever

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Abstract

The relationship between regional adipose tissue (AT) distribution and metabolic diseases has led to an interest in quantifying regional Glycerol (GLY) kinetics. We studied regional GLY kinetics in 6 male subjects (age 3613 yr). An abdominal vein draining subcutaneous (SQ) adipose tissue (AT) and a radial artery were cannulated. Four microdialysis probes were inserted into interstitial SQ AT. A primed-constant infusion of [H5]glycerol was infused for 120 min and abdominal AT blood flow was determined by 133 xenon washout. GLY tracentracee ratio in the artery (8.86±0.9%) was greater than in the vein (2.89±0.43%) (P<0.05) which was greater than in interstitial fluid (1.5210.3%) (P<0.05). Plasma GLY concentrations in abdominal vein (164±21mM) was higher than in the artery (51±6mM) (P<0.01). A plateau in GLY isotope enrichment was achieved by 45 minutes of infusion in both artery and abdominal vein samples. Tracer balance across adipose tissue was not significantly different from zero. Values for regional GLY production calculated by tracer methodology and by arteriovenous balance techniques were similar (0.211.04 and 0.221.04 nmol/100 g/min, respectively) with good correlation between the two methods (slope = 1.1, R = 0.72; P<0.05). We conclude that (1) a primed-constant infusion of GLY tracer reaches isotopic steady state by 45 min, (2) no net extraction of GLY tracer occurs across AT, (3) GLY in AT interstitial fluid should be considered a distinct pool, and (4) Regina] GLY kinetics in AT can be described by at least a 3-compartment model consisting of arterial, venous, and interstitial pools.

Original languageEnglish
Pages (from-to)A520
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

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    Klein, S., Coppack, S., Chinkes, D., & Villanueva-Mever, J. (1996). Regional transendothelial Glycerol kinetics in humans. FASEB Journal, 10(3), A520.