TY - JOUR
T1 - Regional Differences in mRNA and lncRNA Expression Profiles in Non-Failing Human Atria and Ventricles
AU - Johnson, Eric K.
AU - Matkovich, Scot J.
AU - Nerbonne, Jeanne M.
N1 - Funding Information:
This work was made possible by the resources provided by the Translational Cardiovascular Biobank and Repository (TCBR) at Washington University Medical School, supported by the Washington University Institute for Clinical and Translational Sciences (ICTS), recipient of a Clinical and Translational Sciences Award (UL1 RR024992) from the National Institutes of Health (NIH) National Center for Research Resources, the Barnes-Jewish Hospital Foundation and the Richard J. Wilkinson Trust. The authors also thank Drs. Michael K. Pasque for expertise and assistance with cardiac tissue acquisition. This work was supported by the National Heart, Lung and Blood Institute of the NIH (R01 HL-034161 and R01 HL-066388 to JMN), the NIH National Center for Research Resources (UL1 RR024992 to the ICTS) and the American Heart Association (Individual Postdoctoral Fellowship to EKJ). EKJ was also supported in part by an institutional Training Grant (T32-HL007081) from the National Heart Lung and Blood Institute of the NIH. None of these funding sources were involved in study design, data collection, data analyses, data interpretation, manuscript preparation or the decision to submit this article for publication.
Publisher Copyright:
© 2018, The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - The four chambers of the human heart play distinct roles in the maintenance of normal cardiac function, and are differentially affected by inherited/acquired cardiovascular disease. To probe the molecular determinants of these functional differences, we examined mRNA and lncRNA expression profiles in the left (LA) and right (RA) atria, the left (LV) and right (RV) ventricles, and the interventricular septum (IVS) of non-failing human hearts (N = 8). Analysis of paired atrial and ventricular samples (n = 40) identified 5,747 mRNAs and 2,794 lncRNAs that were differentially (>1.5 fold; FDR < 0.05) expressed. The largest differences were observed in comparisons between the atrial (RA/LA) and ventricular (RV/LV/IVS) samples. In every case (e.g., LA vs LV, LA vs RV, etc.), >2,300 mRNAs and >1,200 lncRNAs, corresponding to 17–28% of the total transcripts, were differentially expressed. Heterogeneities in mRNA/lncRNA expression profiles in the LA and RA, as well as in the LV, RV and IVS, were also revealed, although the numbers of differentially expressed transcripts were substantially smaller. Gender differences in mRNA and lncRNA expression profiles were also evident in non-failing human atria and ventricles. Gene ontology classification of differentially expressed gene sets revealed chamber-specific enrichment of numerous signaling pathways.
AB - The four chambers of the human heart play distinct roles in the maintenance of normal cardiac function, and are differentially affected by inherited/acquired cardiovascular disease. To probe the molecular determinants of these functional differences, we examined mRNA and lncRNA expression profiles in the left (LA) and right (RA) atria, the left (LV) and right (RV) ventricles, and the interventricular septum (IVS) of non-failing human hearts (N = 8). Analysis of paired atrial and ventricular samples (n = 40) identified 5,747 mRNAs and 2,794 lncRNAs that were differentially (>1.5 fold; FDR < 0.05) expressed. The largest differences were observed in comparisons between the atrial (RA/LA) and ventricular (RV/LV/IVS) samples. In every case (e.g., LA vs LV, LA vs RV, etc.), >2,300 mRNAs and >1,200 lncRNAs, corresponding to 17–28% of the total transcripts, were differentially expressed. Heterogeneities in mRNA/lncRNA expression profiles in the LA and RA, as well as in the LV, RV and IVS, were also revealed, although the numbers of differentially expressed transcripts were substantially smaller. Gender differences in mRNA and lncRNA expression profiles were also evident in non-failing human atria and ventricles. Gene ontology classification of differentially expressed gene sets revealed chamber-specific enrichment of numerous signaling pathways.
UR - http://www.scopus.com/inward/record.url?scp=85053413207&partnerID=8YFLogxK
U2 - 10.1038/s41598-018-32154-2
DO - 10.1038/s41598-018-32154-2
M3 - Article
C2 - 30224797
AN - SCOPUS:85053413207
SN - 2045-2322
VL - 8
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 13919
ER -