Regional differences in glycoconjugates of intestinal M cells in mice: Potential targets for mucosal vaccines

P. J. Giannasca, K. T. Giannasca, P. Falk, Jeffrey Gordon, M. R. Neutra

Research output: Contribution to journalArticle

160 Scopus citations

Abstract

We have used a panel of lectins and antibodies to describe the composition of complex carbohydrates associated with M cells in various regions of the intestinal tract of adult BALB/c mice. The fucose-specific lectin Ulex europaeus agglutinin type I (UEA I) is a marker of M cells in the small intestine and recognized M cells at an early stage of differentiation. Subpopulations of M cells in a single follicle-associated epithelium (FAE) could be distinguished by different fucose-specific probes. Certain lectins revealed that M cells have basal processes that extend into the underlying lymphoid tissue. Colonic and rectal M cells display glycosylation patterns distinct from M cells of Peyer's patches and are characterized by terminal galactose. UEA I selectively adhered to Peyer's patch M cells in mucosal explants and in ligated intestinal loops in vivo. The lectin was taken up into endocytic vesicles and transported to the intra-epithelial pocket and other domains of the basolateral membrane. Thus M cell-specific glycoconjugates could serve as 'receptors' for targeting of lectin-antigen conjugates to the mucosal immune system.

Original languageEnglish
Pages (from-to)G1108-G1121
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume267
Issue number6 30-6
StatePublished - Dec 1 1994

Keywords

  • host-microbial interactions
  • intestinal epithelium
  • lectins
  • mucosal immunity

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