TY - JOUR
T1 - Regeneration associated transcriptional signature of retinal microglia and macrophages
AU - Mitchell, Diana M.
AU - Sun, Chi
AU - Hunter, Samuel S.
AU - New, Daniel D.
AU - Stenkamp, Deborah L.
N1 - Funding Information:
Funding for this work was provided by an Idaho INBRE Technology Access Grant under an Institutional Development Award (IDeA) from NIH NIGMS award #P20GM103408 (DMM), start-up funds from the University of Idaho (DMM), NIH R21EY026814 (DLS), and data collection and analyses performed by the IBEST Genomics Resources Core at the University of Idaho were supported in part by NIH COBRE grant P30GM103324. We are grateful to Ann Norton (Director of the University of Idaho Optical Imaging Core), University of Oregon for Illumina sequencing, Melissa Oatley of the WSU Center for Reproductive Biology FACS core, Dr. Michael Redd (University of Utah) for the L-plastin antibody, the Zebrafish International Resource Center (ZIRC) for the mpeg1:GFP transgenic line, Ruth Frey and Anna Lovel for zebrafish care and husbandry, Zachary Blume for technical assistance, and Dr. Peter Fuerst (University of Idaho) for critical review of a previous version of the manuscript.
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Zebrafish have the remarkable capacity to regenerate retinal neurons following a variety of damage paradigms. Following initial tissue insult and a period of cell death, a proliferative phase ensues that generates neuronal progenitors, which ultimately regenerate damaged neurons. Recent work has revealed that Müller glia are the source of regenerated neurons in zebrafish. However, the roles of another important class of glia present in the retina, microglia, during this regenerative phase remain elusive. Here, we examine retinal tissue and perform QuantSeq. 3′mRNA sequencing/transcriptome analysis to reveal localization and putative functions, respectively, of mpeg1 expressing cells (microglia/macrophages) during Müller glia-mediated regeneration, corresponding to a time of progenitor proliferation and production of new neurons. Our results indicate that in this regenerative state, mpeg1-expressing cells are located in regions containing regenerative Müller glia and are likely engaged in active vesicle trafficking. Further, mpeg1+ cells congregate at and around the optic nerve head. Our transcriptome analysis reveals several novel genes not previously described in microglia. This dataset represents the first report, to our knowledge, to use RNA sequencing to probe the microglial transcriptome in such context, and therefore provides a resource towards understanding microglia/macrophage function during successful retinal (and central nervous tissue) regeneration.
AB - Zebrafish have the remarkable capacity to regenerate retinal neurons following a variety of damage paradigms. Following initial tissue insult and a period of cell death, a proliferative phase ensues that generates neuronal progenitors, which ultimately regenerate damaged neurons. Recent work has revealed that Müller glia are the source of regenerated neurons in zebrafish. However, the roles of another important class of glia present in the retina, microglia, during this regenerative phase remain elusive. Here, we examine retinal tissue and perform QuantSeq. 3′mRNA sequencing/transcriptome analysis to reveal localization and putative functions, respectively, of mpeg1 expressing cells (microglia/macrophages) during Müller glia-mediated regeneration, corresponding to a time of progenitor proliferation and production of new neurons. Our results indicate that in this regenerative state, mpeg1-expressing cells are located in regions containing regenerative Müller glia and are likely engaged in active vesicle trafficking. Further, mpeg1+ cells congregate at and around the optic nerve head. Our transcriptome analysis reveals several novel genes not previously described in microglia. This dataset represents the first report, to our knowledge, to use RNA sequencing to probe the microglial transcriptome in such context, and therefore provides a resource towards understanding microglia/macrophage function during successful retinal (and central nervous tissue) regeneration.
UR - http://www.scopus.com/inward/record.url?scp=85063047493&partnerID=8YFLogxK
U2 - 10.1038/s41598-019-41298-8
DO - 10.1038/s41598-019-41298-8
M3 - Article
C2 - 30886241
AN - SCOPUS:85063047493
SN - 2045-2322
VL - 9
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 4768
ER -