Regeneration across preserved peripheral nerve grafts

Peter J. Evans, Susan E. Mackinnon, Timothy J. Best, Judith A. Wade, David C. Awerbuck, Akira P. Makino, Daniel A. Hunter, Rajiv Midha

Research output: Contribution to journalArticle

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Abstract

The potential to store nerve grafts for a prolonged period of time was assessed in a rat sciatic nerve model. Three‐centimeter syngeneic nerve grafts were stored in Belzer/University of Wisconsin cold storage solution at different temperatures (5°C, 22°C, or 37°C) for varying time periods (6 h, 24 h, or 3 weeks) prior to transplantation. Functional assessment using serial walking track analyses revealed no difference between storage times and temperatures. At 14 months postengraftment, the conduction velocities and the number of myelinated fibers that had regenerated across all grafts stored at 5°C for all time periods tested were superior to grafts stored at either 22°C or 37°C. Nerve grafts stored for up to 3 weeks at 5°C acted as effective conduits for proximal regenerating fibers and resulted in histologic, electrophysiologic, and functional results equivalent to fresh nerve grafts. Nerve graft storage may be applicable to nerve allografts and potentially provide allograft material that requires reduced or no associated host immunosuppression.© 1995 John Wiley & Sons, Inc.

Original languageEnglish
Pages (from-to)1128-1138
Number of pages11
JournalMuscle & Nerve
Volume18
Issue number10
DOIs
StatePublished - Oct 1995

Keywords

  • electrophysiology
  • nerve grafting
  • nerve regeneration
  • nerve transplantation
  • walking track

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    Evans, P. J., Mackinnon, S. E., Best, T. J., Wade, J. A., Awerbuck, D. C., Makino, A. P., Hunter, D. A., & Midha, R. (1995). Regeneration across preserved peripheral nerve grafts. Muscle & Nerve, 18(10), 1128-1138. https://doi.org/10.1002/mus.880181009