Reevaluation of Drosophila melanogaster's neuronal circadian pacemakers reveals new neuronal classes

Orie Thomas Shafer, Charlotte Helfrich-Förster, Susan Christine Portia Renn, Paul H. Taghert

Research output: Contribution to journalArticle

130 Scopus citations

Abstract

In the brain of the fly Drosophila melanogaster, ∼150 clock-neurons are organized to synchronize and maintain behavioral rhythms, but the physiological and neurochemical bases of their interactions are largely unknown. Here we reevaluate the cellular properties of these pacemakers by application of a novel genetic reporter and several phenotypic markers. First, we describe an enhancer trap marker called R32 that specifically reveals several previously undescribed aspects of the fly's central neuronal pacemakers. We find evidence for a previously unappreciated class of neuronal pacemakers, the lateral posterior neurons (LPNs), and establish anatomical, molecular, and developmental criteria to establish a subclass within the dorsal neuron 1 (DN1) group of pacemakers. Furthermore, we show that the neuropeptide IPNamide is specifically expressed by this DN1 subclass. These observations implicate IPNamide as a second candidate circadian transmitter in the Drosophila brain. Finally, we present molecular and anatomical evidence for unrecognized phenotypic diversity within each of four established classes of clock neurons.

Original languageEnglish
Pages (from-to)180-193
Number of pages14
JournalJournal of Comparative Neurology
Volume498
Issue number2
DOIs
StatePublished - Sep 10 2006

Keywords

  • Circadian clock
  • Drosophila
  • Glass
  • IPNamide
  • Neuropeptides
  • PDF receptor
  • nplp1

Fingerprint Dive into the research topics of 'Reevaluation of Drosophila melanogaster's neuronal circadian pacemakers reveals new neuronal classes'. Together they form a unique fingerprint.

  • Cite this