TY - JOUR
T1 - Reduced inflammatory mediator expression by pre-reperfusion infusion into ischemic territory in rats
T2 - A real-time polymerase chain reaction analysis
AU - Ding, Yuchuan
AU - Young, Chen N.
AU - Li, Jie
AU - Luan, Xiaodong
AU - McAllister, James P.
AU - Clark, Justin D.
AU - Diaz, Fernando G.
N1 - Funding Information:
We are grateful to Dr Qin Lai for his help in the statistical analysis and to Mr Yandong Zhou, MS for his help in the preparation of the manuscript. Drs Alan P. Hudson and Herve C. Gerard are also appreciated for their help in the real-time PCR study. This work was supported partially by an American Heart Association Midwest Affiliate Grant in Aid and Wayne State University Research Stimulation Fund to Y.D.
PY - 2003/12/26
Y1 - 2003/12/26
N2 - The aim in this study was to investigate if our new experimental model for stroke therapy, flushing the ischemic territory with saline prior to reperfusion, could reduce overexpression of inflammatory mediators during reperfusion. Stroke in Sprague-Dawley rats (n=24) was induced by a 2-h middle cerebral artery occlusion using a novel intraluminal hollow filament. Prior to reperfusion, 12 of the ischemic rats received 6 ml isotonic saline at 37°C infused into the ischemic area through the filament. Expression of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and intercellular adhesion molecule 1 (ICAM-1) mRNA was analyzed by real-time reverse transcriptase-polymerase chain reaction (real-time RT-PCR). A significant overexpression (9-26 fold) of the genes encoding TNF-α, IL-1β and ICAM-1 in ischemic rats was found during early reperfusion without flushing at 6 and 12 h. This increase was significantly reduced at both 6 and 12 h post-reperfusion as a result of saline flushing.
AB - The aim in this study was to investigate if our new experimental model for stroke therapy, flushing the ischemic territory with saline prior to reperfusion, could reduce overexpression of inflammatory mediators during reperfusion. Stroke in Sprague-Dawley rats (n=24) was induced by a 2-h middle cerebral artery occlusion using a novel intraluminal hollow filament. Prior to reperfusion, 12 of the ischemic rats received 6 ml isotonic saline at 37°C infused into the ischemic area through the filament. Expression of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and intercellular adhesion molecule 1 (ICAM-1) mRNA was analyzed by real-time reverse transcriptase-polymerase chain reaction (real-time RT-PCR). A significant overexpression (9-26 fold) of the genes encoding TNF-α, IL-1β and ICAM-1 in ischemic rats was found during early reperfusion without flushing at 6 and 12 h. This increase was significantly reduced at both 6 and 12 h post-reperfusion as a result of saline flushing.
KW - Cerebral ischemia
KW - Intercellular adhesion molecule 1
KW - Interleukin 1β
KW - Reperfusion damage
KW - Tumor necrosis factor α
UR - http://www.scopus.com/inward/record.url?scp=0344665739&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2003.09.055
DO - 10.1016/j.neulet.2003.09.055
M3 - Article
C2 - 14665409
AN - SCOPUS:0344665739
SN - 0304-3940
VL - 353
SP - 173
EP - 176
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -