Reduced inflammatory mediator expression by pre-reperfusion infusion into ischemic territory in rats: A real-time polymerase chain reaction analysis

Yuchuan Ding, Chen N. Young, Jie Li, Xiaodong Luan, James P. McAllister, Justin D. Clark, Fernando G. Diaz

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The aim in this study was to investigate if our new experimental model for stroke therapy, flushing the ischemic territory with saline prior to reperfusion, could reduce overexpression of inflammatory mediators during reperfusion. Stroke in Sprague-Dawley rats (n=24) was induced by a 2-h middle cerebral artery occlusion using a novel intraluminal hollow filament. Prior to reperfusion, 12 of the ischemic rats received 6 ml isotonic saline at 37°C infused into the ischemic area through the filament. Expression of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and intercellular adhesion molecule 1 (ICAM-1) mRNA was analyzed by real-time reverse transcriptase-polymerase chain reaction (real-time RT-PCR). A significant overexpression (9-26 fold) of the genes encoding TNF-α, IL-1β and ICAM-1 in ischemic rats was found during early reperfusion without flushing at 6 and 12 h. This increase was significantly reduced at both 6 and 12 h post-reperfusion as a result of saline flushing.

Original languageEnglish
Pages (from-to)173-176
Number of pages4
JournalNeuroscience Letters
Volume353
Issue number3
DOIs
StatePublished - Dec 26 2003

Keywords

  • Cerebral ischemia
  • Intercellular adhesion molecule 1
  • Interleukin 1β
  • Reperfusion damage
  • Tumor necrosis factor α

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