Redefining fundamental concepts of transcription initiation in bacteria

Citlalli Mejía-Almonte, Stephen J.W. Busby, Joseph T. Wade, Jacques van Helden, Adam P. Arkin, Gary D. Stormo, Karen Eilbeck, Bernhard O. Palsson, James E. Galagan, Julio Collado-Vides

Research output: Contribution to journalArticlepeer-review

75 Scopus citations


Despite enormous progress in understanding the fundamentals of bacterial gene regulation, our knowledge remains limited when compared with the number of bacterial genomes and regulatory systems to be discovered. Derived from a small number of initial studies, classic definitions for concepts of gene regulation have evolved as the number of characterized promoters has increased. Together with discoveries made using new technologies, this knowledge has led to revised generalizations and principles. In this Expert Recommendation, we suggest precise, updated definitions that support a logical, consistent conceptual framework of bacterial gene regulation, focusing on transcription initiation. The resulting concepts can be formalized by ontologies for computational modelling, laying the foundation for improved bioinformatics tools, knowledge-based resources and scientific communication. Thus, this work will help researchers construct better predictive models, with different formalisms, that will be useful in engineering, synthetic biology, microbiology and genetics.

Original languageEnglish
Pages (from-to)699-714
Number of pages16
JournalNature Reviews Genetics
Issue number11
StatePublished - Nov 1 2020


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