Recurrent DNA copy number variation in the laboratory mouse

Chris M. Egan, Srinath Sridhar, Michael Wigler, Ira M. Hall

Research output: Contribution to journalArticlepeer-review

115 Scopus citations

Abstract

Different species, populations and individuals vary considerably in the copy number of discrete segments of their genomes. The manner and frequency with which these genetic differences arise over generational time is not well understood. Taking advantage of divergence among lineages sharing a recent common ancestry, we have conducted a genome-wide analysis of spontaneous copy number variation (CNV) in the laboratory mouse. We used high-resolution microarrays to identify 38 CNVs among 14 colonies of the C57BL/6 strain spanning ∼967 generations of inbreeding, and we examined these loci in 12 additional strains. It is clear from our results that many CNVs arise through a highly nonrandom process: 18 of 38 were the product of recurrent mutation, and rates of change varied roughly four orders of magnitude across different loci. Recurrent CNVs are found throughout the genome, affect 43 genes and fluctuate in copy number over mere hundreds of generations, observations that raise questions about their contribution to natural variation.

Original languageEnglish
Pages (from-to)1384-1389
Number of pages6
JournalNature Genetics
Volume39
Issue number11
DOIs
StatePublished - Nov 2007

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