Rectification of acetylcholine-elicited currents in PC12 pheochromocytoma cells

C. K. Ifune, J. H. Steinbach

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


The current-voltage (I-V) relationship for acetylcholine-elicited currents in the rat pheochromocytoma cell line PC12 is nonlinear. Two voltage-dependent processes that could account for the whole-cell current rectification were examined, receptor channel gating and single receptor channel permeation. We found that both factors are involved in the rectification of the whole-cell currents. The voltage dependence of channel gating determines the shape of the I-V curve at negative potentials. The single-channel I-V relationship is inwardly rectifying and largely responsible for the characteristic shape of the whole-cell I-V curve at positive potentials. The rectification of the single-channel currents is produced by the voltage-dependent block of outward currents by intracellular Mg2+ ions.

Original languageEnglish
Pages (from-to)4794-4798
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number12
StatePublished - 1990


  • current-voltage relationship
  • magnesium block
  • neuronal nicotinic acetylcholine receptor


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