TY - JOUR
T1 - Reconstructing subsurface electrical wave orientation from cardiac epi-fluorescence recordings
T2 - Monte Carlo versus diffusion approximation
AU - Hyatt, Christopher J.
AU - Zemlin, Christian W.
AU - Smith, Rebecca M.
AU - Matiukas, Arvydas
AU - Pertsov, Arkady M.
AU - Bernus, Olivier
PY - 2008/9/1
Y1 - 2008/9/1
N2 - The development of voltage-sensitive dyes has revolutionized cardiac electrophysiology and made optical imaging of cardiac electrical activity possible. Photon diffusion models coupled to electrical excitation models have been successful in qualitatively predicting the shape of the optical action potential and its dependence on subsurface electrical wave orientation. However, the accuracy of the diffusion equation in the visible range, especially for thin tissue preparations, remains unclear. Here, we compare diffusion and Monte Carlo (MC) based models and we investigate the role of tissue thickness. All computational results are compared to experimental data obtained from intact guinea pig hearts. We show that the subsurface volume contributing to the epi-fluorescence signal extends deeper in the tissue when using MC models, resulting in longer optical upstroke durations which are in better agreement with experiments. The optical upstroke morphology, however, strongly correlates to the subsurface propagation direction independent of the model and is consistent with our experimental observations.
AB - The development of voltage-sensitive dyes has revolutionized cardiac electrophysiology and made optical imaging of cardiac electrical activity possible. Photon diffusion models coupled to electrical excitation models have been successful in qualitatively predicting the shape of the optical action potential and its dependence on subsurface electrical wave orientation. However, the accuracy of the diffusion equation in the visible range, especially for thin tissue preparations, remains unclear. Here, we compare diffusion and Monte Carlo (MC) based models and we investigate the role of tissue thickness. All computational results are compared to experimental data obtained from intact guinea pig hearts. We show that the subsurface volume contributing to the epi-fluorescence signal extends deeper in the tissue when using MC models, resulting in longer optical upstroke durations which are in better agreement with experiments. The optical upstroke morphology, however, strongly correlates to the subsurface propagation direction independent of the model and is consistent with our experimental observations.
UR - http://www.scopus.com/inward/record.url?scp=51149121423&partnerID=8YFLogxK
U2 - 10.1364/OE.16.013758
DO - 10.1364/OE.16.013758
M3 - Article
C2 - 18772987
AN - SCOPUS:51149121423
SN - 1094-4087
VL - 16
SP - 13758
EP - 13772
JO - Optics Express
JF - Optics Express
IS - 18
ER -