Recombinant rat surfactant-associated protein D inhibits human T lymphocyte proliferation and IL-2 production

Paul J. Borron, Erika C. Crouch, James F. Lewis, Jo Rae Wright, Fred Possmayer, Laurence J. Fraher

Research output: Contribution to journalArticlepeer-review

149 Scopus citations

Abstract

Components of the airspace-lining material may contribute to the local regulation of immune function within the lung. We report here that recombinant rat pulmonary surfactant-associated protein D (SP-D) inhibits the lectin- and anti-CD3-stimulated proliferation of human PBMCs. Inhibition was associated with a decreased production of IL-2, and the addition of human rIL-2 blocked the inhibitory action of SP-D. These effects were not inhibited by maltose, indicating that the inhibitory activity was not dependent upon the lectin activity of SP-D. Studies employing mutant SP-D lacking N-linked sugars or defective in multimerization further indicated that inhibition was not dependent upon cellular interactions with the N-linked oligosaccharide on SP-D or the oligomerization of trimeric SP-D subunits. Although a peptide containing an inverted DGR showed similar IL-2-dependent effects on anti- CD3-stimulated proliferation, deletion of the conserved DGRDGR sequence near the amino-terminal end of the collagen domain did not decrease the suppressive activity of SP-D. We hypothesize that SP-D can dampen lymphocyte responses to exogenous stimuli and protect the lung against collateral immune-mediated damage.

Original languageEnglish
Pages (from-to)4599-4603
Number of pages5
JournalJournal of Immunology
Volume161
Issue number9
StatePublished - Nov 1 1998

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