Reclamation of ampicillin sensitivity for the genetic manipulation of Legionella pneumophila

Molly C. Sutherland; Joseph P. Vogel

doi:10.1128/AEM.00669-12

Reclamation of ampicillin sensitivity for the genetic manipulation of Legionella pneumophila

Molly C. Sutherland
, Joseph P. Vogel

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Research on Legionella pneumophila, the causative agent of Legionnaires' disease, has been hampered due to the lack of selectable markers for genetic manipulation. We report the construction of a mutant strain of L. pneumophila lacking loxA, a chromosomally encoded Β-lactamase, that has enhanced sensitivity to ampicillin. Also described are a method for converting Legionella strains to ampicillin sensitivity and conditions for utilizing bla as a selectable marker.

Original language	English
Pages (from-to)	5457-5459
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	15
DOIs	https://doi.org/10.1128/AEM.00669-12
State	Published - Aug 2012

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title = "Reclamation of ampicillin sensitivity for the genetic manipulation of Legionella pneumophila",

abstract = "Research on Legionella pneumophila, the causative agent of Legionnaires' disease, has been hampered due to the lack of selectable markers for genetic manipulation. We report the construction of a mutant strain of L. pneumophila lacking loxA, a chromosomally encoded Β-lactamase, that has enhanced sensitivity to ampicillin. Also described are a method for converting Legionella strains to ampicillin sensitivity and conditions for utilizing bla as a selectable marker.",

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AB - Research on Legionella pneumophila, the causative agent of Legionnaires' disease, has been hampered due to the lack of selectable markers for genetic manipulation. We report the construction of a mutant strain of L. pneumophila lacking loxA, a chromosomally encoded Β-lactamase, that has enhanced sensitivity to ampicillin. Also described are a method for converting Legionella strains to ampicillin sensitivity and conditions for utilizing bla as a selectable marker.

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Reclamation of ampicillin sensitivity for the genetic manipulation of Legionella pneumophila

Abstract

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