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Research on Legionella pneumophila, the causative agent of Legionnaires' disease, has been hampered due to the lack of selectable markers for genetic manipulation. We report the construction of a mutant strain of L. pneumophila lacking loxA, a chromosomally encoded Β-lactamase, that has enhanced sensitivity to ampicillin. Also described are a method for converting Legionella strains to ampicillin sensitivity and conditions for utilizing bla as a selectable marker.

Original languageEnglish
Pages (from-to)5457-5459
Number of pages3
JournalApplied and Environmental Microbiology
Issue number15
StatePublished - Aug 2012


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