Reclamation of ampicillin sensitivity for the genetic manipulation of Legionella pneumophila

Molly C. Sutherland, Joseph P. Vogel

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Research on Legionella pneumophila, the causative agent of Legionnaires' disease, has been hampered due to the lack of selectable markers for genetic manipulation. We report the construction of a mutant strain of L. pneumophila lacking loxA, a chromosomally encoded Β-lactamase, that has enhanced sensitivity to ampicillin. Also described are a method for converting Legionella strains to ampicillin sensitivity and conditions for utilizing bla as a selectable marker.

Original languageEnglish
Pages (from-to)5457-5459
Number of pages3
JournalApplied and Environmental Microbiology
Volume78
Issue number15
DOIs
StatePublished - Aug 1 2012

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