TY - JOUR
T1 - Reclamation of ampicillin sensitivity for the genetic manipulation of Legionella pneumophila
AU - Sutherland, Molly C.
AU - Vogel, Joseph P.
PY - 2012/8
Y1 - 2012/8
N2 - Research on Legionella pneumophila, the causative agent of Legionnaires' disease, has been hampered due to the lack of selectable markers for genetic manipulation. We report the construction of a mutant strain of L. pneumophila lacking loxA, a chromosomally encoded Β-lactamase, that has enhanced sensitivity to ampicillin. Also described are a method for converting Legionella strains to ampicillin sensitivity and conditions for utilizing bla as a selectable marker.
AB - Research on Legionella pneumophila, the causative agent of Legionnaires' disease, has been hampered due to the lack of selectable markers for genetic manipulation. We report the construction of a mutant strain of L. pneumophila lacking loxA, a chromosomally encoded Β-lactamase, that has enhanced sensitivity to ampicillin. Also described are a method for converting Legionella strains to ampicillin sensitivity and conditions for utilizing bla as a selectable marker.
UR - http://www.scopus.com/inward/record.url?scp=84866166524&partnerID=8YFLogxK
U2 - 10.1128/AEM.00669-12
DO - 10.1128/AEM.00669-12
M3 - Article
C2 - 22635996
AN - SCOPUS:84866166524
SN - 0099-2240
VL - 78
SP - 5457
EP - 5459
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 15
ER -