TY - JOUR
T1 - Recent advances in lineage tracing for the kidney
AU - Muto, Yoshiharu
AU - Humphreys, Benjamin D.
N1 - Publisher Copyright:
© 2021 International Society of Nephrology
PY - 2021/12
Y1 - 2021/12
N2 - Lineage tracing was originally developed by developmental biologists to identify all progeny of a single cell during morphogenesis. More recently this approach has been applied to other fields, including organ homeostasis and recovery from injury. Modern lineage tracing techniques typically rely on reporter gene expression induced by cell-specific DNA recombination. There have been important scientific advances in the last 10 years that have impacted lineage tracing approaches, including intersectional genetics, optical clearing techniques, and the use of sequencing-based genomic lineage tracing. The latter combines CRISPR-Cas9–based genetic scarring with single-cell RNA-sequencing that, in theory, could allow comprehensive reconstruction of a lineage tree for an entire organism. This review summarizes recent advances in lineage tracing technologies and outlines potential applications for kidney research.
AB - Lineage tracing was originally developed by developmental biologists to identify all progeny of a single cell during morphogenesis. More recently this approach has been applied to other fields, including organ homeostasis and recovery from injury. Modern lineage tracing techniques typically rely on reporter gene expression induced by cell-specific DNA recombination. There have been important scientific advances in the last 10 years that have impacted lineage tracing approaches, including intersectional genetics, optical clearing techniques, and the use of sequencing-based genomic lineage tracing. The latter combines CRISPR-Cas9–based genetic scarring with single-cell RNA-sequencing that, in theory, could allow comprehensive reconstruction of a lineage tree for an entire organism. This review summarizes recent advances in lineage tracing technologies and outlines potential applications for kidney research.
KW - CRISPR-Cas9
KW - Cre recombinase
KW - lineage analysis
UR - http://www.scopus.com/inward/record.url?scp=85116014175&partnerID=8YFLogxK
U2 - 10.1016/j.kint.2021.05.040
DO - 10.1016/j.kint.2021.05.040
M3 - Short survey
C2 - 34217781
AN - SCOPUS:85116014175
SN - 0085-2538
VL - 100
SP - 1179
EP - 1184
JO - Kidney International
JF - Kidney International
IS - 6
ER -