Recent advances in genome editing and creation of genetically modified pigs

James R. Butler; Joseph M. Ladowski; Gregory R. Martens; Matthew Tector; A. Joseph Tector

doi:10.1016/j.ijsu.2015.07.684

Recent advances in genome editing and creation of genetically modified pigs

James R. Butler, Joseph M. Ladowski, Gregory R. Martens, Matthew Tector, A. Joseph Tector

Section of Transplant Surgery

Research output: Contribution to journal › Review article › peer-review

50 Scopus citations

Abstract

The field of xenotransplantation is benefiting greatly from recent advances in genetic engineering. The efficiency and pace with which new model animals are being created has dramatically sped progress towards clinical relevance. Endonuclease-driven genome editing now allows for the efficient generation of targeted genetic alterations. Herein we review the available methods of genetic engineering that have been successfully employed to create genetically modified pigs.

Original language	English
Pages (from-to)	217-222
Number of pages	6
Journal	International Journal of Surgery
Volume	23
DOIs	https://doi.org/10.1016/j.ijsu.2015.07.684
State	Published - Nov 1 2015

Keywords

Endonuclease
Homologous recombination
Off-target
TALEN CRISPR/Cas9
Zinc finger nuclease

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10.1016/j.ijsu.2015.07.684

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title = "Recent advances in genome editing and creation of genetically modified pigs",

abstract = "The field of xenotransplantation is benefiting greatly from recent advances in genetic engineering. The efficiency and pace with which new model animals are being created has dramatically sped progress towards clinical relevance. Endonuclease-driven genome editing now allows for the efficient generation of targeted genetic alterations. Herein we review the available methods of genetic engineering that have been successfully employed to create genetically modified pigs.",

keywords = "Endonuclease, Homologous recombination, Off-target, TALEN CRISPR/Cas9, Zinc finger nuclease",

author = "Butler, {James R.} and Ladowski, {Joseph M.} and Martens, {Gregory R.} and Matthew Tector and Tector, {A. Joseph}",

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AU - Ladowski, Joseph M.

AU - Martens, Gregory R.

AU - Tector, Matthew

AU - Tector, A. Joseph

PY - 2015/11/1

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AB - The field of xenotransplantation is benefiting greatly from recent advances in genetic engineering. The efficiency and pace with which new model animals are being created has dramatically sped progress towards clinical relevance. Endonuclease-driven genome editing now allows for the efficient generation of targeted genetic alterations. Herein we review the available methods of genetic engineering that have been successfully employed to create genetically modified pigs.

KW - Endonuclease

KW - Homologous recombination

KW - Off-target

KW - TALEN CRISPR/Cas9

KW - Zinc finger nuclease

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DO - 10.1016/j.ijsu.2015.07.684

M3 - Review article

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VL - 23

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EP - 222

JO - International Journal of Surgery

JF - International Journal of Surgery

ER -

Recent advances in genome editing and creation of genetically modified pigs

Abstract

Keywords

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