Central venous catheter (CVC) -related infections are a significant source of preventable morbidity and mortality. To reduce CVC-related infections, we developed a web-based educational intervention. Video records of care provider performance of CVC insertion were used in task analysis and in expert review of performance as part of a needs analysis. Stakeholder opinions of compliance to recommended sterile practices were elicited. Based on these data, we developed an online training course for residents on recommended sterile practices during CVC insertion. The course contained short video clips from actual patient care demonstrating common breaks in recommended sterile practices. We propose reasons why the video-based course was effective in promoting compliant behavior, and reasons training influences attitudes toward sterile practices.