Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors

A. De; J. Van Nieuwkasteele; E. T. Carlen; A. Van Den Berg

doi:10.1109/Transducers.2013.6627386

Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors

A. De, J. Van Nieuwkasteele, E. T. Carlen, A. Van Den Berg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Scopus citations

Abstract

Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be K_A7×10⁶ M¹ in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be 10¹² molecules/cm² with a radiolabeled DNA-P³² assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a differential measurement.

Original language	English
Title of host publication	2013 Transducers and Eurosensors XXVII
Subtitle of host publication	The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Pages	2795-2798
Number of pages	4
DOIs	https://doi.org/10.1109/Transducers.2013.6627386
State	Published - 2013
Event	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 - Barcelona, Spain Duration: Jun 16 2013 → Jun 20 2013

Publication series

Name	2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Conference

Conference	2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013
Country/Territory	Spain
City	Barcelona
Period	06/16/13 → 06/20/13

Keywords

hybridization kinetics
Nano-ISFET
PNA
silicon nanowire

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10.1109/Transducers.2013.6627386

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De, A., Van Nieuwkasteele, J., Carlen, E. T., & Van Den Berg, A. (2013). Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 (pp. 2795-2798). Article 6627386 (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013). https://doi.org/10.1109/Transducers.2013.6627386

De, A. ; Van Nieuwkasteele, J. ; Carlen, E. T. et al. / Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors. 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. pp. 2795-2798 (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013).

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title = "Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors",

abstract = "Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be KA7×106 M1 in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be 1012 molecules/cm2 with a radiolabeled DNA-P32 assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a differential measurement.",

keywords = "hybridization kinetics, Nano-ISFET, PNA, silicon nanowire",

author = "A. De and {Van Nieuwkasteele}, J. and Carlen, {E. T.} and {Van Den Berg}, A.",

year = "2013",

doi = "10.1109/Transducers.2013.6627386",

language = "English",

isbn = "9781467359818",

series = "2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013",

pages = "2795--2798",

booktitle = "2013 Transducers and Eurosensors XXVII",

note = "2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013 ; Conference date: 16-06-2013 Through 20-06-2013",

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De, A, Van Nieuwkasteele, J, Carlen, ET & Van Den Berg, A 2013, Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors. in 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013., 6627386, 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, pp. 2795-2798, 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013, Barcelona, Spain, 06/16/13. https://doi.org/10.1109/Transducers.2013.6627386

Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors. / De, A.; Van Nieuwkasteele, J.; Carlen, E. T. et al.
2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 2795-2798 6627386 (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors

AU - De, A.

AU - Van Nieuwkasteele, J.

AU - Carlen, E. T.

AU - Van Den Berg, A.

PY - 2013

Y1 - 2013

N2 - Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be KA7×106 M1 in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be 1012 molecules/cm2 with a radiolabeled DNA-P32 assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a differential measurement.

AB - Real-time electronic detection of peptide nucleic acid (PNA):DNA hybridization is demonstrated with silicon nanowire (Si-NW) biosensors. A stable baseline platform is crucial for obtaining the Si-NW response for biosensing applications. An integrated Si-NW auto-sampler is demonstrated and used for kinetic molecular binding measurements. The equilibrium association constant is determined to be KA7×106 M1 in 1 mM ionic strength buffer. The surface density of hybridized DNA is determined to be 1012 molecules/cm2 with a radiolabeled DNA-P32 assay. A 30× reduction in the measurement drift in Si-NW sensor response is obtained from 1.6 nA/hr to 0.04 nA/hr by using a differential measurement.

KW - hybridization kinetics

KW - Nano-ISFET

KW - PNA

KW - silicon nanowire

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DO - 10.1109/Transducers.2013.6627386

M3 - Conference contribution

AN - SCOPUS:84891677977

SN - 9781467359818

T3 - 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

SP - 2795

EP - 2798

BT - 2013 Transducers and Eurosensors XXVII

T2 - 2013 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013

Y2 - 16 June 2013 through 20 June 2013

ER -

De A, Van Nieuwkasteele J, Carlen ET, Van Den Berg A. Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors. In 2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013. 2013. p. 2795-2798. 6627386. (2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013). doi: 10.1109/Transducers.2013.6627386

Real-time measurements of PNA:DNA hybridization kinetics with silicon nanowire biosensors

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