TY - JOUR
T1 - Real-time analysis of glucose metabolism by microscopy
AU - Piston, David W.
AU - Knobel, Susan M.
N1 - Funding Information:
The authors thank Drs James May and Per Arkhammer for their help with some of the experiments shown here, and Mr Guangtao Ying for his help in constructing the two-photon excitation microscope. This work has been supported by grants to DWP from the NIH (DK53434) and NSF (BRI 98-70163), and by the Vanderbilt Cell Imaging Resource (supported by CA68485 and DK20593).
PY - 1999/12/1
Y1 - 1999/12/1
N2 - Glucose metabolism has traditionally been assayed via biochemical means. Fluorescence monitoring of NAD(P)H levels has provided a noninvasive method to assay glucose metabolism in cells and tissues. However, these measurements have traditionally been of low resolution (no subcellular information) because of limitations imposed by optical and cellular photodamage problems. The recent advent of two-photon excitation microscopy as a dependable tool for biological research has opened the possibility of real-time, high- resolution analysis of glucose metabolism in living cells. Such measurements have the potential to provide subcellular information from intact tissue that cannot be obtained by other techniques.
AB - Glucose metabolism has traditionally been assayed via biochemical means. Fluorescence monitoring of NAD(P)H levels has provided a noninvasive method to assay glucose metabolism in cells and tissues. However, these measurements have traditionally been of low resolution (no subcellular information) because of limitations imposed by optical and cellular photodamage problems. The recent advent of two-photon excitation microscopy as a dependable tool for biological research has opened the possibility of real-time, high- resolution analysis of glucose metabolism in living cells. Such measurements have the potential to provide subcellular information from intact tissue that cannot be obtained by other techniques.
UR - http://www.scopus.com/inward/record.url?scp=0033485944&partnerID=8YFLogxK
U2 - 10.1016/S1043-2760(99)00204-0
DO - 10.1016/S1043-2760(99)00204-0
M3 - Review article
AN - SCOPUS:0033485944
SN - 1043-2760
VL - 10
SP - 413
EP - 417
JO - Trends in Endocrinology and Metabolism
JF - Trends in Endocrinology and Metabolism
IS - 10
ER -