RCADiA: Simple automation platform for comparative multidimensional protein identification technology

Aaron O. Bailey, Timothy M. Miller, Meng Qiu Dong, Christine Vande Velde, Don W. Cleveland, John R. Yates

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Multidimensional liquid chromatography in combination with tandem mass spectrometry has been used to analyze a variety of biological structures including protein complexes. Incorporating this approach with autosampling devices presents a number of problems including decreased sensitivity due to exposure to extra surfaces, carryover from run to run, and increased dead volume. We developed a device, termed Radial Column Array for Distribution and Automation (RCADiA), to automate multiple MuDPIT experiments while eliminating many of these problems and maintaining a high resolution and sensitive analysis. The design, which places each sample downstream of any common fluid path, presents a low risk of carryover between successive analyses. Beyond the convenience of automation, the RCADiA platform also produces data of similar quality to the standard method of performing individual MuDPIT experiments. We demonstrate this device by performing a comparative analysis of mitochondria enriched from rat liver and spinal cord.

Original languageEnglish
Pages (from-to)6410-6418
Number of pages9
JournalAnalytical Chemistry
Volume79
Issue number16
DOIs
StatePublished - Aug 15 2007
Externally publishedYes

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