Rate of anterograde axonal transport of [125I]wheat germ agglutinin from retina to optic tectum in the chick

Todd P. Margolis; Jennifer H. LaVail

doi:10.1016/0006-8993(81)90760-5

Rate of anterograde axonal transport of [¹²⁵I]wheat germ agglutinin from retina to optic tectum in the chick

Todd P. Margolis, Jennifer H. LaVail

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We have investigated the rate of anterograde axonal transport of affinity-purified [¹²⁵I]wheat germ agglutinin (WGA) in chick retinal ganglion cells. By 2 lines of evidence, we found the rate to be 22-44 mm/day. This rate is slower than that reported for many other endogenously synthesized proteins or for horseradish peroxidase, the only other exogenously supplied protein studied as an anterograde transported marker. This difference in rate of transport of [¹²⁵I]WGA may reflect a novel pathway important in membrane recycling.

Original language	English
Pages (from-to)	218-223
Number of pages	6
Journal	Brain Research
Volume	229
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(81)90760-5
State	Published - Dec 14 1981

Keywords

anterograde axonal transport
chick
retinal ganglion cell
wheat germ agglutinin

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10.1016/0006-8993(81)90760-5

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title = "Rate of anterograde axonal transport of [125I]wheat germ agglutinin from retina to optic tectum in the chick",

abstract = "We have investigated the rate of anterograde axonal transport of affinity-purified [125I]wheat germ agglutinin (WGA) in chick retinal ganglion cells. By 2 lines of evidence, we found the rate to be 22-44 mm/day. This rate is slower than that reported for many other endogenously synthesized proteins or for horseradish peroxidase, the only other exogenously supplied protein studied as an anterograde transported marker. This difference in rate of transport of [125I]WGA may reflect a novel pathway important in membrane recycling.",

keywords = "anterograde axonal transport, chick, retinal ganglion cell, wheat germ agglutinin",

author = "Margolis, {Todd P.} and LaVail, {Jennifer H.}",

note = "Funding Information: This study was supportedin part by grantsf rom the National Instituteso f Health,P ublic Health ServiceR 01 NS 13533a nd T32 GM 07618.",

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AU - Margolis, Todd P.

AU - LaVail, Jennifer H.

N1 - Funding Information: This study was supportedin part by grantsf rom the National Instituteso f Health,P ublic Health ServiceR 01 NS 13533a nd T32 GM 07618.

PY - 1981/12/14

Y1 - 1981/12/14

N2 - We have investigated the rate of anterograde axonal transport of affinity-purified [125I]wheat germ agglutinin (WGA) in chick retinal ganglion cells. By 2 lines of evidence, we found the rate to be 22-44 mm/day. This rate is slower than that reported for many other endogenously synthesized proteins or for horseradish peroxidase, the only other exogenously supplied protein studied as an anterograde transported marker. This difference in rate of transport of [125I]WGA may reflect a novel pathway important in membrane recycling.

AB - We have investigated the rate of anterograde axonal transport of affinity-purified [125I]wheat germ agglutinin (WGA) in chick retinal ganglion cells. By 2 lines of evidence, we found the rate to be 22-44 mm/day. This rate is slower than that reported for many other endogenously synthesized proteins or for horseradish peroxidase, the only other exogenously supplied protein studied as an anterograde transported marker. This difference in rate of transport of [125I]WGA may reflect a novel pathway important in membrane recycling.

KW - anterograde axonal transport

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KW - retinal ganglion cell

KW - wheat germ agglutinin

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JO - Brain Research

JF - Brain Research

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ER -

Rate of anterograde axonal transport of [¹²⁵I]wheat germ agglutinin from retina to optic tectum in the chick

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Keywords

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