TY - JOUR
T1 - Rat prothrombin
T2 - Purification, characterization, and activation
AU - Grant, G. A.
AU - Suttie, J. W.
N1 - Funding Information:
’ Research supported by the College of Agricultural and Life Sciences, University of Wisconsin-Madison, and in part by Grant No. AM-14881 from the National Institutes of Health, USPHS, and in part by National Institutes of Health Training Grant No. GM-00236 BCH. ’ Present address: Department Chemistry, Division of Biology ences, Washington University, of Biological and Biomedical Sci-St. Louis, MO. 63110.
PY - 1976/10
Y1 - 1976/10
N2 - Prothrombin has been purified from rat plasma and its properties compared to prothrombin isolated from other species. The molecular weight, amino acid composition, and amino-terminal sequence of rat prothrombin are similar to human and bovine prothrombin. Rat prothrombin binds to phospholipid in the presence of calcium ions, and calcium-binding measurements indicate that it may bind somewhat more calcium than does bovine prothrombin. The proteolytic cleavage of purified rat prothrombin by Factor Xa or thrombin yields the same peptides that are formed from similar proteolysis of bovine prothrombin. Factor V and phospholipid were shown to enhance the rate of Factor Xa and calcium ion generation of thrombin from rat prothrombin.
AB - Prothrombin has been purified from rat plasma and its properties compared to prothrombin isolated from other species. The molecular weight, amino acid composition, and amino-terminal sequence of rat prothrombin are similar to human and bovine prothrombin. Rat prothrombin binds to phospholipid in the presence of calcium ions, and calcium-binding measurements indicate that it may bind somewhat more calcium than does bovine prothrombin. The proteolytic cleavage of purified rat prothrombin by Factor Xa or thrombin yields the same peptides that are formed from similar proteolysis of bovine prothrombin. Factor V and phospholipid were shown to enhance the rate of Factor Xa and calcium ion generation of thrombin from rat prothrombin.
UR - http://www.scopus.com/inward/record.url?scp=0017194623&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(76)90209-5
DO - 10.1016/0003-9861(76)90209-5
M3 - Article
C2 - 984850
AN - SCOPUS:0017194623
SN - 0003-9861
VL - 176
SP - 650
EP - 662
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -