TY - JOUR
T1 - Rat pancreatic lipase and two related proteins
T2 - enzymatic properties and mRNA expression during development
AU - Payne, R. M.
AU - Sims, H. F.
AU - Jennens, M. L.
AU - Lowe, M. E.
PY - 1994
Y1 - 1994
N2 - We report the cDNA sequences of rat colipase, rat pancreatic lipase (rPL), and a rat pancreatic lipase-related protein (rPLRP). Comparison to the human PLRP cDNA suggests that the isolated clone encodes rPLRP-2. Both cDNA and a third cDNA encoding rPLRP-1 are secreted from Sf9 cells infected with recombinant baculovirus. rPL and rPLRP-2 hydrolyze triolein, 8.0 and 4.4 μmol · min-1 · μg-1, respectively. They are inhibited by bile salts, and activity is restored by (pro)colipase. PLRP-1 has barely detectable activity against triolein, even with (pro)colipase present. The pattern of mRNA expression during development in the rat reveals that all mRNA are low in the fetal rat pancreas. Both PLRP mRNA rise just before birth to a maximum 12 h after birth. They fall to low levels in the adult. In contrast, the PL mRNA is low at birth and rises rapidly during the suckling-veanling transition. In conclusion, the rat has at least three genes encoding different lipases, and these related genes have separate regulatory controls.
AB - We report the cDNA sequences of rat colipase, rat pancreatic lipase (rPL), and a rat pancreatic lipase-related protein (rPLRP). Comparison to the human PLRP cDNA suggests that the isolated clone encodes rPLRP-2. Both cDNA and a third cDNA encoding rPLRP-1 are secreted from Sf9 cells infected with recombinant baculovirus. rPL and rPLRP-2 hydrolyze triolein, 8.0 and 4.4 μmol · min-1 · μg-1, respectively. They are inhibited by bile salts, and activity is restored by (pro)colipase. PLRP-1 has barely detectable activity against triolein, even with (pro)colipase present. The pattern of mRNA expression during development in the rat reveals that all mRNA are low in the fetal rat pancreas. Both PLRP mRNA rise just before birth to a maximum 12 h after birth. They fall to low levels in the adult. In contrast, the PL mRNA is low at birth and rises rapidly during the suckling-veanling transition. In conclusion, the rat has at least three genes encoding different lipases, and these related genes have separate regulatory controls.
KW - baculovirus
KW - protein expression
KW - triglycerides
UR - http://www.scopus.com/inward/record.url?scp=0028307251&partnerID=8YFLogxK
U2 - 10.1152/ajpgi.1994.266.5.g914
DO - 10.1152/ajpgi.1994.266.5.g914
M3 - Article
C2 - 8203536
AN - SCOPUS:0028307251
SN - 0193-1857
VL - 266
SP - G914-G921
JO - American Journal of Physiology - Gastrointestinal and Liver Physiology
JF - American Journal of Physiology - Gastrointestinal and Liver Physiology
IS - 5 29-5
ER -