Rat-brain Na,K-ATPase beta-chain gene: primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells.

R. W. Mercer; J. W. Schneider; A. Savitz; J. Emanuel; E. J. Benz; R. Levenson

doi:10.1128/MCB.6.11.3884

Rat-brain Na,K-ATPase beta-chain gene: primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells.

R. W. Mercer, J. W. Schneider, A. Savitz, J. Emanuel, E. J. Benz, R. Levenson

Research output: Contribution to journal › Article

86 Scopus citations

Abstract

We deduced the complete amino acid sequence of the rat brain Na,K-ATPase beta-subunit from cDNA. The rat brain beta-subunit exhibits a high degree of primary sequence and secondary structural homology with the human and Torpedo beta-subunit polypeptides. Analysis of rat tissue RNA reveals that the beta-subunit gene encodes four separate mRNA species which are expressed in a tissue-specific fashion. In ouabain-resistant HeLa C+ cells, beta-subunit DNA sequences are amplified (approximately 20-fold) and beta-subunit mRNAs are overproduced relative to levels in parental HeLa cells. These results suggest that the beta-subunit plays an important role in Na,K-ATPase structure-function and in the mechanism underlying cellular resistance to the cardiac glycosides.

Original language	English
Pages (from-to)	3884-3890
Number of pages	7
Journal	Molecular and cellular biology
Volume	6
Issue number	11
DOIs	https://doi.org/10.1128/MCB.6.11.3884
State	Published - Nov 1986
Externally published	Yes

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Mercer, R. W., Schneider, J. W., Savitz, A., Emanuel, J., Benz, E. J., & Levenson, R. (1986). Rat-brain Na,K-ATPase beta-chain gene: primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells. Molecular and cellular biology, 6(11), 3884-3890. https://doi.org/10.1128/MCB.6.11.3884

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abstract = "We deduced the complete amino acid sequence of the rat brain Na,K-ATPase beta-subunit from cDNA. The rat brain beta-subunit exhibits a high degree of primary sequence and secondary structural homology with the human and Torpedo beta-subunit polypeptides. Analysis of rat tissue RNA reveals that the beta-subunit gene encodes four separate mRNA species which are expressed in a tissue-specific fashion. In ouabain-resistant HeLa C+ cells, beta-subunit DNA sequences are amplified (approximately 20-fold) and beta-subunit mRNAs are overproduced relative to levels in parental HeLa cells. These results suggest that the beta-subunit plays an important role in Na,K-ATPase structure-function and in the mechanism underlying cellular resistance to the cardiac glycosides.",

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