TY - JOUR
T1 - Rat-brain Na,K-ATPase beta-chain gene
T2 - primary structure, tissue-specific expression, and amplification in ouabain-resistant HeLa C+ cells.
AU - Mercer, R. W.
AU - Schneider, J. W.
AU - Savitz, A.
AU - Emanuel, J.
AU - Benz, E. J.
AU - Levenson, R.
PY - 1986/11
Y1 - 1986/11
N2 - We deduced the complete amino acid sequence of the rat brain Na,K-ATPase beta-subunit from cDNA. The rat brain beta-subunit exhibits a high degree of primary sequence and secondary structural homology with the human and Torpedo beta-subunit polypeptides. Analysis of rat tissue RNA reveals that the beta-subunit gene encodes four separate mRNA species which are expressed in a tissue-specific fashion. In ouabain-resistant HeLa C+ cells, beta-subunit DNA sequences are amplified (approximately 20-fold) and beta-subunit mRNAs are overproduced relative to levels in parental HeLa cells. These results suggest that the beta-subunit plays an important role in Na,K-ATPase structure-function and in the mechanism underlying cellular resistance to the cardiac glycosides.
AB - We deduced the complete amino acid sequence of the rat brain Na,K-ATPase beta-subunit from cDNA. The rat brain beta-subunit exhibits a high degree of primary sequence and secondary structural homology with the human and Torpedo beta-subunit polypeptides. Analysis of rat tissue RNA reveals that the beta-subunit gene encodes four separate mRNA species which are expressed in a tissue-specific fashion. In ouabain-resistant HeLa C+ cells, beta-subunit DNA sequences are amplified (approximately 20-fold) and beta-subunit mRNAs are overproduced relative to levels in parental HeLa cells. These results suggest that the beta-subunit plays an important role in Na,K-ATPase structure-function and in the mechanism underlying cellular resistance to the cardiac glycosides.
UR - http://www.scopus.com/inward/record.url?scp=0022820964&partnerID=8YFLogxK
U2 - 10.1128/MCB.6.11.3884
DO - 10.1128/MCB.6.11.3884
M3 - Article
C2 - 3025616
AN - SCOPUS:0022820964
VL - 6
SP - 3884
EP - 3890
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 11
ER -