TY - JOUR
T1 - Rat Apolipoprotein A-IV
T2 - Application of Computational Methods for Studying the Structure, Function, and Evolution of a Protein
AU - Boguski, Mark S.
AU - Elshourbagy, Nabil A.
AU - Taylor, John M.
AU - Gordon, Jeffrey I.
PY - 1986/1/1
Y1 - 1986/1/1
N2 - ApoA-IV is one of the major protein components of rat chylomicrons and HDL and is also associated with chylomicrons in humans and dogs; however, the precise metabolic function of this protein remains obscure. This chapter presents a computer program appropriate to study protein and nucleic acid sequences, the critical interpretation of the results that can lead to specific and experimentally testable predictions of a protein's structure and function, and may yield insights into its evolution and the location of its gene. The chapter demonstrates that rat apoA-IV bears a striking structural similarity to human apoA-I. Statistical analyses of homologies between apolipoproteins A-I, A-IV, and E show that all three sequences diverged from a common ancestral gene. Assuming that the genes for these proteins had evolved as a result of a series of intra- and intergenic unequal cross-overs, it is likely that their genetic loci were at one time linked. Given this fact, the existence of other genes or pseudogenes based upon this repeated sequence motif is a distinct possibility.
AB - ApoA-IV is one of the major protein components of rat chylomicrons and HDL and is also associated with chylomicrons in humans and dogs; however, the precise metabolic function of this protein remains obscure. This chapter presents a computer program appropriate to study protein and nucleic acid sequences, the critical interpretation of the results that can lead to specific and experimentally testable predictions of a protein's structure and function, and may yield insights into its evolution and the location of its gene. The chapter demonstrates that rat apoA-IV bears a striking structural similarity to human apoA-I. Statistical analyses of homologies between apolipoproteins A-I, A-IV, and E show that all three sequences diverged from a common ancestral gene. Assuming that the genes for these proteins had evolved as a result of a series of intra- and intergenic unequal cross-overs, it is likely that their genetic loci were at one time linked. Given this fact, the existence of other genes or pseudogenes based upon this repeated sequence motif is a distinct possibility.
UR - http://www.scopus.com/inward/record.url?scp=0022556118&partnerID=8YFLogxK
U2 - 10.1016/0076-6879(86)28104-5
DO - 10.1016/0076-6879(86)28104-5
M3 - Article
C2 - 3088393
AN - SCOPUS:0022556118
VL - 128
SP - 753
EP - 773
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
IS - C
ER -