TY - GEN
T1 - Rare-cell enrichment using closed-loop operation of spiral microfluidics
AU - Ryu, Hyunryul
AU - Choi, Kyungyong
AU - Qu, Yanyan
AU - Kwon, Taehong
AU - Lee, Janet
AU - Han, Jongyoon
PY - 2016
Y1 - 2016
N2 - In this research, we demonstrate a novel operating method of spiral microfluidics to enrich rarecell from the patient-derived sample, even if unit single pass separation step cannot provide adequate separation resolution. By feeding the output stream of the separation back to original sample, and running continuously with high flow processing rate, one can achieve high preconcentration, recovery and purity of target cells. We demonstrated this method in two clinically relevant cell separation challenges; sputum and blood separation.
AB - In this research, we demonstrate a novel operating method of spiral microfluidics to enrich rarecell from the patient-derived sample, even if unit single pass separation step cannot provide adequate separation resolution. By feeding the output stream of the separation back to original sample, and running continuously with high flow processing rate, one can achieve high preconcentration, recovery and purity of target cells. We demonstrated this method in two clinically relevant cell separation challenges; sputum and blood separation.
KW - Cell separation
KW - Patient sample preparation
KW - Recirculatory inertial microfluidics
UR - http://www.scopus.com/inward/record.url?scp=85014131065&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85014131065
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 683
EP - 684
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Y2 - 9 October 2016 through 13 October 2016
ER -