TY - JOUR
T1 - Rapid separation of anionic oligosaccharide species by high performance liquid chromatography
AU - Baenziger, Jacques U.
AU - Natowicz, Marvin
N1 - Funding Information:
1 This investigation was supported by Grant RO 1 CA 21923 awarded by the National Cancer Institute, DHEW, and Grant PCM-7911486 awarded by the National Science Foundation. J. U. B. is a recipient of a Research Career Development Award, Grant 1 K04 CAO0671, from the National Cancer Institute, DHEW. M. N. is a Medical Scientist Trainee supported by Grant GM07200 from the National Institute of General Medical Sciences. This study was also supported by the following companies: Brown & Williamson Tobacco Corporation; Philip Morris Incorporated: R. J. Reynolds Tobacco Company; and United States Tobacco Company.
PY - 1981/4
Y1 - 1981/4
N2 - We have developed a method for the rapid separation of anionic oligosaccharide species by high-performance liquid chromatography utilizing a MicroPak AX-10 ion-exchange column (Varian Associates) with the mobile phase consisting of 25-500 mm KH2PO4, pH4.0. Separation of oligosaccharides bearing zero, one, two, three or four sialic acid residues requires less than 45 min. Oligosaccharides containing mannose-6-PO4 moieties in monoester or diester linkage can also be analyzed in this system. Preparative separations of as much as 20 mg of oligosaccharide can be accomplished in a single chromatographic analysis with quantitative yields of oligosaccharide. This method should prove useful for the rapid isolation and characterization of anionic oligosaccharide species.
AB - We have developed a method for the rapid separation of anionic oligosaccharide species by high-performance liquid chromatography utilizing a MicroPak AX-10 ion-exchange column (Varian Associates) with the mobile phase consisting of 25-500 mm KH2PO4, pH4.0. Separation of oligosaccharides bearing zero, one, two, three or four sialic acid residues requires less than 45 min. Oligosaccharides containing mannose-6-PO4 moieties in monoester or diester linkage can also be analyzed in this system. Preparative separations of as much as 20 mg of oligosaccharide can be accomplished in a single chromatographic analysis with quantitative yields of oligosaccharide. This method should prove useful for the rapid isolation and characterization of anionic oligosaccharide species.
UR - http://www.scopus.com/inward/record.url?scp=0019793586&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(81)90305-5
DO - 10.1016/0003-2697(81)90305-5
M3 - Article
C2 - 7258650
AN - SCOPUS:0019793586
SN - 0003-2697
VL - 112
SP - 357
EP - 361
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -