TY - JOUR
T1 - Rapid screening and identification of compounds with DNA-binding activity from Folium Citri Reticulatae using on-line HPLC-DAD-MSn coupled with a post column fluorescence detection system
AU - Fu, Qingrong
AU - Zhang, Cangman
AU - Lin, Zongtao
AU - Sun, Hongyang
AU - Liang, Yi
AU - Jiang, Haixiu
AU - Song, Zhiling
AU - Wang, Hong
AU - Chen, Shizhong
N1 - Funding Information:
This work was supported by the National Science and Technology Major Project: Safety Testing Technology of the New Drug of TCM and the Standard Research (NO. 2014ZX09304307-001-011 ). The authors wish to thank Ms. Kinsie Arnst in the Department of Pharmaceutical Sciences, University of Tennessee Health Science Center for proofreading the manuscript.
Publisher Copyright:
© 2015 Elsevier Ltd. All rights reserved.
PY - 2016/7/13
Y1 - 2016/7/13
N2 - To study the interactions between natural compounds and deoxyribonucleic acid (DNA), a method has been established combining a high-performance liquid chromatography-diode array detector-multi-stage mass spectrometer with a fluorescence detector (HPLC-DAD-MSn-FLD). The FLD was used to monitor fluorescence intensity of the ethidium bromide-DNA (EB-DNA) complex when a compound separated by HPLC was introduced. This novel method was used to simultaneously obtain the HPLC fingerprint, UV spectra, MSn fragments and DNA-binding activity profile of various components in Folium Citri Reticulatae. As a result, 35 compounds were identified, of which 25 were found in the extract of Folium Citri Reticulatae for the first time, and 33 compounds showed DNA-binding activities, with the most active being feruloylhexaric and p-coumaroylhexaric acids. In addition, the precision, stability and reproducibility of this method were validated by two positive controls, quercetin and hesperidin. This new on-line method is accurate, precise and reliable for further high-throughput screening of DNA-binding compounds from food samples and other complex matrices.
AB - To study the interactions between natural compounds and deoxyribonucleic acid (DNA), a method has been established combining a high-performance liquid chromatography-diode array detector-multi-stage mass spectrometer with a fluorescence detector (HPLC-DAD-MSn-FLD). The FLD was used to monitor fluorescence intensity of the ethidium bromide-DNA (EB-DNA) complex when a compound separated by HPLC was introduced. This novel method was used to simultaneously obtain the HPLC fingerprint, UV spectra, MSn fragments and DNA-binding activity profile of various components in Folium Citri Reticulatae. As a result, 35 compounds were identified, of which 25 were found in the extract of Folium Citri Reticulatae for the first time, and 33 compounds showed DNA-binding activities, with the most active being feruloylhexaric and p-coumaroylhexaric acids. In addition, the precision, stability and reproducibility of this method were validated by two positive controls, quercetin and hesperidin. This new on-line method is accurate, precise and reliable for further high-throughput screening of DNA-binding compounds from food samples and other complex matrices.
KW - DNA-binding activity
KW - Feruloylhexaric acids
KW - Flavonoids
KW - Folium Citri Reticulatae
KW - HPLC-DAD-MS-EB-DNA-FLD
KW - p-Coumaroylhexaric acids
UR - http://www.scopus.com/inward/record.url?scp=84937053304&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2015.07.009
DO - 10.1016/j.foodchem.2015.07.009
M3 - Article
C2 - 26304344
AN - SCOPUS:84937053304
SN - 0308-8146
VL - 192
SP - 250
EP - 259
JO - Food Chemistry
JF - Food Chemistry
ER -