Rapid kinetics of the interaction between daunomycin and drug-sensitive or drug-resistant P388 leukemia cells

Florentina Soto, Jaume M. Canaves, Jose M. Gonzalez-Ros, Jose A. Ferragut

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The initial stages of the interaction of daunomycin (DNM) with drug-sensitive (P388/S) and drug-resistant (P388/100) cells have been defined by a rapid kinetics stopped-flow procedure. The process can be described by two kinetic components. The faster component accounts for rapid occupation of cell surface sites by DNM, as supported by experiments with liposomes with different surface charge. On the other hand, the effect of verapamil in the assays, suggests that the slower component is involved in the transport of the drug into the cells. Our observations are consistent with a loss in the control of the passive permeability to the drugs in the drug-resistant tumor cells.

Original languageEnglish
Pages (from-to)119-123
Number of pages5
JournalFEBS Letters
Volume301
Issue number1
DOIs
StatePublished - Apr 13 1992
Externally publishedYes

Keywords

  • P388 cell
  • Rapid kinetics. Anthracycline resistance

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