Rapid kinetics of the interaction between daunomycin and drug-sensitive or drug-resistant P388 leukemia cells

Florentina Soto; Jaume M. Canaves; Jose M. Gonzalez-Ros; Jose A. Ferragut

doi:10.1016/0014-5793(92)80223-4

Rapid kinetics of the interaction between daunomycin and drug-sensitive or drug-resistant P388 leukemia cells

Florentina Soto, Jaume M. Canaves, Jose M. Gonzalez-Ros, Jose A. Ferragut

Department of Ophthalmology and Visual Sciences

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The initial stages of the interaction of daunomycin (DNM) with drug-sensitive (P388/S) and drug-resistant (P388/100) cells have been defined by a rapid kinetics stopped-flow procedure. The process can be described by two kinetic components. The faster component accounts for rapid occupation of cell surface sites by DNM, as supported by experiments with liposomes with different surface charge. On the other hand, the effect of verapamil in the assays, suggests that the slower component is involved in the transport of the drug into the cells. Our observations are consistent with a loss in the control of the passive permeability to the drugs in the drug-resistant tumor cells.

Original language	English
Pages (from-to)	119-123
Number of pages	5
Journal	FEBS Letters
Volume	301
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(92)80223-4
State	Published - Apr 13 1992

Keywords

P388 cell
Rapid kinetics. Anthracycline resistance

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10.1016/0014-5793(92)80223-4

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title = "Rapid kinetics of the interaction between daunomycin and drug-sensitive or drug-resistant P388 leukemia cells",

abstract = "The initial stages of the interaction of daunomycin (DNM) with drug-sensitive (P388/S) and drug-resistant (P388/100) cells have been defined by a rapid kinetics stopped-flow procedure. The process can be described by two kinetic components. The faster component accounts for rapid occupation of cell surface sites by DNM, as supported by experiments with liposomes with different surface charge. On the other hand, the effect of verapamil in the assays, suggests that the slower component is involved in the transport of the drug into the cells. Our observations are consistent with a loss in the control of the passive permeability to the drugs in the drug-resistant tumor cells.",

keywords = "P388 cell, Rapid kinetics. Anthracycline resistance",

author = "Florentina Soto and Canaves, {Jaume M.} and Gonzalez-Ros, {Jose M.} and Ferragut, {Jose A.}",

note = "Funding Information: nrkrto~~,(cll~Tehirs, ?wcorrklr ,hsa:s been partly supported by grants from the DGICYT PB90-0564o nd the European Economic Community CT91-0666. F.S. and J.M.C. arc rccipiemso fprcdoctornl fellow-shipsf rom the {\textquoteleft}Conscllcriad c Cuhura, Educacioi cicnciu, Gcncralhat Valcnciana{\textquoteright} and {\textquoteleft}Programa de Formation dc Personal Invcshgador{\textquoteright}, respectivelyW. e thank Dr. A. Campos for his assistancew ith the Epics instrument.",

year = "1992",

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doi = "10.1016/0014-5793(92)80223-4",

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AU - Soto, Florentina

AU - Canaves, Jaume M.

AU - Gonzalez-Ros, Jose M.

AU - Ferragut, Jose A.

N1 - Funding Information: nrkrto~~,(cll~Tehirs, ?wcorrklr ,hsa:s been partly supported by grants from the DGICYT PB90-0564o nd the European Economic Community CT91-0666. F.S. and J.M.C. arc rccipiemso fprcdoctornl fellow-shipsf rom the ‘Conscllcriad c Cuhura, Educacioi cicnciu, Gcncralhat Valcnciana’ and ‘Programa de Formation dc Personal Invcshgador’, respectivelyW. e thank Dr. A. Campos for his assistancew ith the Epics instrument.

PY - 1992/4/13

Y1 - 1992/4/13

N2 - The initial stages of the interaction of daunomycin (DNM) with drug-sensitive (P388/S) and drug-resistant (P388/100) cells have been defined by a rapid kinetics stopped-flow procedure. The process can be described by two kinetic components. The faster component accounts for rapid occupation of cell surface sites by DNM, as supported by experiments with liposomes with different surface charge. On the other hand, the effect of verapamil in the assays, suggests that the slower component is involved in the transport of the drug into the cells. Our observations are consistent with a loss in the control of the passive permeability to the drugs in the drug-resistant tumor cells.

AB - The initial stages of the interaction of daunomycin (DNM) with drug-sensitive (P388/S) and drug-resistant (P388/100) cells have been defined by a rapid kinetics stopped-flow procedure. The process can be described by two kinetic components. The faster component accounts for rapid occupation of cell surface sites by DNM, as supported by experiments with liposomes with different surface charge. On the other hand, the effect of verapamil in the assays, suggests that the slower component is involved in the transport of the drug into the cells. Our observations are consistent with a loss in the control of the passive permeability to the drugs in the drug-resistant tumor cells.

KW - P388 cell

KW - Rapid kinetics. Anthracycline resistance

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ER -

Rapid kinetics of the interaction between daunomycin and drug-sensitive or drug-resistant P388 leukemia cells

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Keywords

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