Rapid communication: Enalapril decreases nuclear factor κB activation in the kidney with ureteral obstruction

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Abstract

The transcription factor nuclear factor kappa B (NF-κB) controls a number of genes associated with tissue inflammation and has been shown to be activated in the kidney with ureteral obstruction. In this investigation, we further explored NF-κB activation in the kidney cortex of rats with unilateral ureteral obstruction. Electrophoretic mobility shift assays combined with antibody supershift/depletion demonstrated that NF-κB subunits p50, p52, c-rel, p65 (RelA) and RelB were all activated. Immunocytochemical analysis using an antibody to the p50 subunit demonstrated activation occurring predominantly in nuclei of tubular cells. Treatment of animals with unilateral ureteral obstruction with an oral ACE inhibitor significantly decreased NF-κB activation. This suggests that the antiinflammatory effect of ACE inhibitors in renal disease is in part due to a blunting of NF-κB activation.

Original languageEnglish
Pages (from-to)926-933
Number of pages8
JournalKidney International
Volume52
Issue number4
DOIs
StatePublished - 1997

Keywords

  • ACE inhibition
  • DNA-binding protein
  • Enalapril
  • Inflammation
  • Nuclear factor κB
  • Obstruction
  • Transcription factors

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