Raman microspectroscopy differentiates perinatal pathogens on ex vivo infected human fetal membrane tissues

Oscar D. Ayala, Ryan S. Doster, Shannon D. Manning, Christine M. O'Brien, David M. Aronoff, Jennifer A. Gaddy, Anita Mahadevan-Jansen

Research output: Contribution to journalLetterpeer-review

3 Scopus citations

Abstract

Streptococcus agalactiae, also known as Group B Streptococcus (GBS), is a major cause of chorioamnionitis and neonatal sepsis. This study evaluates Raman spectroscopy (RS) to identify spectral characteristics of infection and differentiate GBS from Escherichia coli and Staphylococcus aureus during ex vivo infection of human fetal membrane tissues. Unique spectral features were identified from colonies grown on agar and infected fetal membrane tissues. Multinomial logistic regression analysis accurately identified GBS infected tissues with 100.0% sensitivity and 88.9% specificity. Together, these findings support further investigation into the use of RS as an emerging microbiologic diagnostic tool and intrapartum screening test for GBS carriage.

Original languageEnglish
Article numbere201800449
JournalJournal of Biophotonics
Volume12
Issue number9
DOIs
StatePublished - Sep 1 2019

Keywords

  • GBS
  • Group B Streptococcus
  • Raman spectroscopy
  • Streptococcus agalactiae
  • biofilms
  • chorioamnionitis

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