Radioprotection by DMSO against the biological effects of incorporated radionuclides in vivo. Comparison with other radioprotectors and evidence for indirect action of Auger electrons

S. Murty Goddu; Venkat R. Narra; Ravi S. Harapanhalli; Roger W. Howell; Dandamudi V. Rao

doi:10.3109/02841869609104044

Radioprotection by DMSO against the biological effects of incorporated radionuclides in vivo. Comparison with other radioprotectors and evidence for indirect action of Auger electrons

S. Murty Goddu, Venkat R. Narra, Ravi S. Harapanhalli, Roger W. Howell, Dandamudi V. Rao

Division of Medical Physics

Research output: Contribution to journal › Conference article › peer-review

22 Scopus citations

Abstract

Dimethyl sulfoxide (DMSO) was studied for its capacity to protect against the biological effects of chronic irradiation by incorporated radionuclides. Spermatogenesis in mice was used as experimental model and spermatogonial cell survival was the biological endpoint. DMSO was injected intratesticularly 4 h prior to a similar injection of the radiochemical and the spermhead survival determined. Iodine-125 was localized in either the cytoplasm (H¹²⁵IPDM) or in the DNA (¹²⁵IUdR) of the testicular cells. Protection was observed against the high-LET type effects of DNA-bound ¹²⁵I as well as the low-LET effects of cytoplasmically localized ¹²⁵I with dose modification factors (DMF) of 3.1 ± 1.0 and 4.4 ± 1.0 respectively. No protection (DMF = 1.1 ± 0.1) was observed against the effects of high-LET 5.3 MeV alpha particles of ²¹⁰Po. The present findings provide supporting evidence that the mechanism responsible for the extreme biological damage caused by DNA-bound Auger emitters is largely radical mediated and therefore indirect in nature.

Original language	English
Pages (from-to)	901-907
Number of pages	7
Journal	Acta Oncologica
Volume	35
Issue number	7
DOIs	https://doi.org/10.3109/02841869609104044
State	Published - 1996
Event	Proceedings of the 1995 3rd International Symposium on Biophysical Aspects of Auger Processes - Lund, Swed Duration: Aug 24 1995 → Aug 25 1995

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@article{3f4cc791a70544788483b50eecc8ae5a,

title = "Radioprotection by DMSO against the biological effects of incorporated radionuclides in vivo. Comparison with other radioprotectors and evidence for indirect action of Auger electrons",

abstract = "Dimethyl sulfoxide (DMSO) was studied for its capacity to protect against the biological effects of chronic irradiation by incorporated radionuclides. Spermatogenesis in mice was used as experimental model and spermatogonial cell survival was the biological endpoint. DMSO was injected intratesticularly 4 h prior to a similar injection of the radiochemical and the spermhead survival determined. Iodine-125 was localized in either the cytoplasm (H125IPDM) or in the DNA (125IUdR) of the testicular cells. Protection was observed against the high-LET type effects of DNA-bound 125I as well as the low-LET effects of cytoplasmically localized 125I with dose modification factors (DMF) of 3.1 ± 1.0 and 4.4 ± 1.0 respectively. No protection (DMF = 1.1 ± 0.1) was observed against the effects of high-LET 5.3 MeV alpha particles of 210Po. The present findings provide supporting evidence that the mechanism responsible for the extreme biological damage caused by DNA-bound Auger emitters is largely radical mediated and therefore indirect in nature.",

author = "Goddu, {S. Murty} and Narra, {Venkat R.} and Harapanhalli, {Ravi S.} and Howell, {Roger W.} and Rao, {Dandamudi V.}",

note = "Funding Information: The authors would like to express their gratitude to Hank F. Kung for providing HIPDM. This work was supported in part by USPHS grant No. CA-32877, New Jersey Cancer Commission Research Fellowship grant Nos. 91-2133-CCR-00 (RSH) and 689-080 (VRN), and UMDNJ Foundation grant No. 26-93.; Proceedings of the 1995 3rd International Symposium on Biophysical Aspects of Auger Processes ; Conference date: 24-08-1995 Through 25-08-1995",

year = "1996",

doi = "10.3109/02841869609104044",

language = "English",

volume = "35",

pages = "901--907",

journal = "Acta Oncologica",

issn = "0284-186X",

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Radioprotection by DMSO against the biological effects of incorporated radionuclides in vivo. Comparison with other radioprotectors and evidence for indirect action of Auger electrons. / Goddu, S. Murty; Narra, Venkat R.; Harapanhalli, Ravi S. et al.
In: Acta Oncologica, Vol. 35, No. 7, 1996, p. 901-907.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Radioprotection by DMSO against the biological effects of incorporated radionuclides in vivo. Comparison with other radioprotectors and evidence for indirect action of Auger electrons

AU - Goddu, S. Murty

AU - Narra, Venkat R.

AU - Harapanhalli, Ravi S.

AU - Howell, Roger W.

AU - Rao, Dandamudi V.

N1 - Funding Information: The authors would like to express their gratitude to Hank F. Kung for providing HIPDM. This work was supported in part by USPHS grant No. CA-32877, New Jersey Cancer Commission Research Fellowship grant Nos. 91-2133-CCR-00 (RSH) and 689-080 (VRN), and UMDNJ Foundation grant No. 26-93.

PY - 1996

Y1 - 1996

N2 - Dimethyl sulfoxide (DMSO) was studied for its capacity to protect against the biological effects of chronic irradiation by incorporated radionuclides. Spermatogenesis in mice was used as experimental model and spermatogonial cell survival was the biological endpoint. DMSO was injected intratesticularly 4 h prior to a similar injection of the radiochemical and the spermhead survival determined. Iodine-125 was localized in either the cytoplasm (H125IPDM) or in the DNA (125IUdR) of the testicular cells. Protection was observed against the high-LET type effects of DNA-bound 125I as well as the low-LET effects of cytoplasmically localized 125I with dose modification factors (DMF) of 3.1 ± 1.0 and 4.4 ± 1.0 respectively. No protection (DMF = 1.1 ± 0.1) was observed against the effects of high-LET 5.3 MeV alpha particles of 210Po. The present findings provide supporting evidence that the mechanism responsible for the extreme biological damage caused by DNA-bound Auger emitters is largely radical mediated and therefore indirect in nature.

AB - Dimethyl sulfoxide (DMSO) was studied for its capacity to protect against the biological effects of chronic irradiation by incorporated radionuclides. Spermatogenesis in mice was used as experimental model and spermatogonial cell survival was the biological endpoint. DMSO was injected intratesticularly 4 h prior to a similar injection of the radiochemical and the spermhead survival determined. Iodine-125 was localized in either the cytoplasm (H125IPDM) or in the DNA (125IUdR) of the testicular cells. Protection was observed against the high-LET type effects of DNA-bound 125I as well as the low-LET effects of cytoplasmically localized 125I with dose modification factors (DMF) of 3.1 ± 1.0 and 4.4 ± 1.0 respectively. No protection (DMF = 1.1 ± 0.1) was observed against the effects of high-LET 5.3 MeV alpha particles of 210Po. The present findings provide supporting evidence that the mechanism responsible for the extreme biological damage caused by DNA-bound Auger emitters is largely radical mediated and therefore indirect in nature.

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AN - SCOPUS:0030461523

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SP - 901

EP - 907

JO - Acta Oncologica

JF - Acta Oncologica

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T2 - Proceedings of the 1995 3rd International Symposium on Biophysical Aspects of Auger Processes

Y2 - 24 August 1995 through 25 August 1995

ER -

Radioprotection by DMSO against the biological effects of incorporated radionuclides in vivo. Comparison with other radioprotectors and evidence for indirect action of Auger electrons

Abstract

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