Radioimmunoassay of 3α-hydroxy-5α-pregnan-20-one in rat and human plasma

Robert H. Purdy; Perry H. Moore; P. Narasimha Rao; Nobuyoshi Hagino; Tatsumi Yamaguchi; Peter Schmidt; David R. Rubinow; A. Leslie Morrow; Steven M. Paul

doi:10.1016/0039-128X(90)90031-6

Radioimmunoassay of 3α-hydroxy-5α-pregnan-20-one in rat and human plasma

Robert H. Purdy, Perry H. Moore, P. Narasimha Rao, Nobuyoshi Hagino, Tatsumi Yamaguchi, Peter Schmidt, David R. Rubinow, A. Leslie Morrow, Steven M. Paul

Washington University School of Medicine

Research output: Contribution to journal › Article › peer-review

204 Scopus citations

Abstract

A radioimmunoassay for measuring 3α-hydroxy-5α-pregnan-20-one in plasma has been developed. Polyclonal antibodies were raised in rabbits against 3α-hydroxy-20-oxo-5α-pregnan-11α-yl carboxymethyl ether coupled to bovine serum albumin. 3α-Hydroxy-5α-pregnan-20-one was purified from ether extracts of plasma by high-performance liquid chromatography. These antibodies were then used for the radioimmunoassay of this centrally active progesterone metabolite in rat and human plasma. 3α-Hydroxy-5α-pregnan-20-one was detected in plasma from female rats on the day ofestrus (2.0 to 9.3 ng/ml) and in the plasma of women during the luteal phase of the menstrual cycle at levels ranging from 0.25 to 2.5 ng/ml. The latter was highly correlated with plasma progesterone levels.

Original language	English
Pages (from-to)	290-296
Number of pages	7
Journal	Steroids
Volume	55
Issue number	7
DOIs	https://doi.org/10.1016/0039-128X(90)90031-6
State	Published - Jul 1990

Keywords

allopregnanolone
plasma RIA
progesterone
steroids

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10.1016/0039-128X(90)90031-6

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@article{882f33d82b8748feaac79705477cc62e,

title = "Radioimmunoassay of 3α-hydroxy-5α-pregnan-20-one in rat and human plasma",

abstract = "A radioimmunoassay for measuring 3α-hydroxy-5α-pregnan-20-one in plasma has been developed. Polyclonal antibodies were raised in rabbits against 3α-hydroxy-20-oxo-5α-pregnan-11α-yl carboxymethyl ether coupled to bovine serum albumin. 3α-Hydroxy-5α-pregnan-20-one was purified from ether extracts of plasma by high-performance liquid chromatography. These antibodies were then used for the radioimmunoassay of this centrally active progesterone metabolite in rat and human plasma. 3α-Hydroxy-5α-pregnan-20-one was detected in plasma from female rats on the day ofestrus (2.0 to 9.3 ng/ml) and in the plasma of women during the luteal phase of the menstrual cycle at levels ranging from 0.25 to 2.5 ng/ml. The latter was highly correlated with plasma progesterone levels.",

keywords = "allopregnanolone, plasma RIA, progesterone, steroids",

author = "Purdy, {Robert H.} and Moore, {Perry H.} and {Narasimha Rao}, P. and Nobuyoshi Hagino and Tatsumi Yamaguchi and Peter Schmidt and Rubinow, {David R.} and {Leslie Morrow}, A. and Paul, {Steven M.}",

note = "Funding Information: This work was supported by Biomedical Research Support Grant 2507RRO6619-2f6ro m the Division of Research Resources, National Institutes of Health, and by a grantf rom the Upjohn Company. The authors are indebted to Dr. William F. Crowley, Jr., for providing the initial sampleso f human plasma, to Dr. A. Burl Henderson for the statistical analysis, and to Josephine Fletcher and S. Christina Klemm for assistance in the preparationo f this manuscript.",

year = "1990",

month = jul,

doi = "10.1016/0039-128X(90)90031-6",

language = "English",

volume = "55",

pages = "290--296",

journal = "Steroids",

issn = "0039-128X",

number = "7",

}

TY - JOUR

T1 - Radioimmunoassay of 3α-hydroxy-5α-pregnan-20-one in rat and human plasma

AU - Purdy, Robert H.

AU - Moore, Perry H.

AU - Narasimha Rao, P.

AU - Hagino, Nobuyoshi

AU - Yamaguchi, Tatsumi

AU - Schmidt, Peter

AU - Rubinow, David R.

AU - Leslie Morrow, A.

AU - Paul, Steven M.

N1 - Funding Information: This work was supported by Biomedical Research Support Grant 2507RRO6619-2f6ro m the Division of Research Resources, National Institutes of Health, and by a grantf rom the Upjohn Company. The authors are indebted to Dr. William F. Crowley, Jr., for providing the initial sampleso f human plasma, to Dr. A. Burl Henderson for the statistical analysis, and to Josephine Fletcher and S. Christina Klemm for assistance in the preparationo f this manuscript.

PY - 1990/7

Y1 - 1990/7

N2 - A radioimmunoassay for measuring 3α-hydroxy-5α-pregnan-20-one in plasma has been developed. Polyclonal antibodies were raised in rabbits against 3α-hydroxy-20-oxo-5α-pregnan-11α-yl carboxymethyl ether coupled to bovine serum albumin. 3α-Hydroxy-5α-pregnan-20-one was purified from ether extracts of plasma by high-performance liquid chromatography. These antibodies were then used for the radioimmunoassay of this centrally active progesterone metabolite in rat and human plasma. 3α-Hydroxy-5α-pregnan-20-one was detected in plasma from female rats on the day ofestrus (2.0 to 9.3 ng/ml) and in the plasma of women during the luteal phase of the menstrual cycle at levels ranging from 0.25 to 2.5 ng/ml. The latter was highly correlated with plasma progesterone levels.

AB - A radioimmunoassay for measuring 3α-hydroxy-5α-pregnan-20-one in plasma has been developed. Polyclonal antibodies were raised in rabbits against 3α-hydroxy-20-oxo-5α-pregnan-11α-yl carboxymethyl ether coupled to bovine serum albumin. 3α-Hydroxy-5α-pregnan-20-one was purified from ether extracts of plasma by high-performance liquid chromatography. These antibodies were then used for the radioimmunoassay of this centrally active progesterone metabolite in rat and human plasma. 3α-Hydroxy-5α-pregnan-20-one was detected in plasma from female rats on the day ofestrus (2.0 to 9.3 ng/ml) and in the plasma of women during the luteal phase of the menstrual cycle at levels ranging from 0.25 to 2.5 ng/ml. The latter was highly correlated with plasma progesterone levels.

KW - allopregnanolone

KW - plasma RIA

KW - progesterone

KW - steroids

UR - http://www.scopus.com/inward/record.url?scp=0025030029&partnerID=8YFLogxK

U2 - 10.1016/0039-128X(90)90031-6

DO - 10.1016/0039-128X(90)90031-6

M3 - Article

C2 - 2120801

AN - SCOPUS:0025030029

SN - 0039-128X

VL - 55

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EP - 296

JO - Steroids

JF - Steroids

IS - 7

ER -

Radioimmunoassay of 3α-hydroxy-5α-pregnan-20-one in rat and human plasma

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Keywords

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