Radioimmunoassay for the detection of antigen-specific IgM, IgG, and IgA in equine sera.

T. P. Rearden, R. F. Sprouse, H. E. Garner

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A radioimmunoassay was developed to discriminate immunoglobulin (Ig) classes specific for the J-5 mutant of Escherichia coli (serotype O:111-B4). Adult horses were periodically inoculated IM with a nonviable suspension of the J-5 mutant emulsified in Freund's incomplete adjuvant. Before and after the horses were inoculated, sera were collected sequentially and examined by radioimmunoassay. Rabbit anti-(horse) Ig and [125I]protein A served as the indicator system. Antigen-specific IgM, IgG, and IgA were observed to follow a classic immune response. The radioimmunoassay offers a valuable tool for the discrimination of circulating, antigen-specific Ig in the horse.

Original languageEnglish
Pages (from-to)294-298
Number of pages5
JournalAmerican journal of veterinary research
Volume43
Issue number2
StatePublished - Feb 1 1982
Externally publishedYes

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