TY - JOUR
T1 - Radioimmunoassay for digoxin. Technic and clinical application
AU - Oliver, G. Charles
AU - Parker, Brent M.
AU - Parker, Charles W.
N1 - Funding Information:
From the Cardiovascular Division and Division of Immunology of the Department of Medicine, Washington University School of Medicine, 4550 Scott Avenue, St. Louis, Missouri 63110. This investigation was supported by U.S. Public Health Service Grants No. 11233 and 11034 from the National Institutes of Health, Bethesda, Maty-land. Requests for reprints should be addressed to Dr. G. Charles Oliver, Cardiology Division, Jewish Hospital, Washing ton University School of Medicine, 216 S. Kingshighway, St. Louis, Missouri 63110. Manuscript received August 3, 1970.
PY - 1971/8
Y1 - 1971/8
N2 - A rapid and sensitive radioimmunoassay for digoxin is described, which is capable of detecting as little as 25 μμg of digoxin. This assay, requiring as little as 0.1 ml of serum or urine, allows measurements of blood or urine digoxin concentration to be made easily within one working day. Patients judged on clinical grounds to have digoxin toxicity had average plasma digoxin levels of 3 mμg/ml. Patients thought to be adequately digitalized had average plasma levels of 1.6 mμg/ml, and those considered to be inadequately digitalized had average levels of 0.5 mμg/ml. These differences are highly significant (p < 0.001). The usefulness of plasma digoxin measurement in the detection and management of digitalis toxicity is discussed, and a role of the immunoassay in studying the pharmacology of digoxin absorption and excretion is briefly indicated.
AB - A rapid and sensitive radioimmunoassay for digoxin is described, which is capable of detecting as little as 25 μμg of digoxin. This assay, requiring as little as 0.1 ml of serum or urine, allows measurements of blood or urine digoxin concentration to be made easily within one working day. Patients judged on clinical grounds to have digoxin toxicity had average plasma digoxin levels of 3 mμg/ml. Patients thought to be adequately digitalized had average plasma levels of 1.6 mμg/ml, and those considered to be inadequately digitalized had average levels of 0.5 mμg/ml. These differences are highly significant (p < 0.001). The usefulness of plasma digoxin measurement in the detection and management of digitalis toxicity is discussed, and a role of the immunoassay in studying the pharmacology of digoxin absorption and excretion is briefly indicated.
UR - http://www.scopus.com/inward/record.url?scp=0015112811&partnerID=8YFLogxK
U2 - 10.1016/0002-9343(71)90237-3
DO - 10.1016/0002-9343(71)90237-3
M3 - Article
C2 - 5095526
AN - SCOPUS:0015112811
SN - 0002-9343
VL - 51
SP - 186
EP - 192
JO - The American journal of medicine
JF - The American journal of medicine
IS - 2
ER -