Radiographic and micro-computed tomographic imaging of lipopolysaccharide- mediated bone resorption

Robert Nason, Dong H. Lee, Jae Y. Jung, Richard A. Chole

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Objectives: Chronic otitis media and cholesteatomas cause hearing loss as a result of bony erosion. This bone resorption is known to be more aggressive when cholesteatomas become infected. The most common organism isolated from both diseases is the gram-negative bacterium Pseudomonas aeruginosa. Lipolysaccharide (LPS), a major virulence factor found in the gram-negative bacterial cell wall, is well known to incite inflammatory bone resorption. The mechanisms underlying this process, however, are poorly understood. In this study, we developed a mouse model of calvarial osteolysis in which resorption was reliably imaged by plain radiography and micro-computed tomography (micro-CT). Methods: A murine calvarial model was developed to study bone resorption induced by P aeruginosa LPS. Calvariae from wild-type and knockout mice used in this model were imaged by plain radiography and micro-CT. Results: A high degree of correlation between plain radiography and micro-CT was identified (R2 = 0.8554). Furthermore, maximal LPS-induced bone resorption required functioning toll-like receptor (TLR) 2, TLR4, and myeloid differentiation factor 88 (MyD88). Conclusions: We have developed a successful model of inflammatory osteolysis in which plain radiography can reliably delineate induced bone resorption. In vivo, we have shown that P aeruginosa LPS signals via TLR2, as well as TLR4 through MyD88.

Original languageEnglish
Pages (from-to)391-396
Number of pages6
JournalAnnals of Otology, Rhinology and Laryngology
Issue number5
StatePublished - May 2009


  • Calvaria
  • Cholesteatoma
  • Chronic otitis media
  • Micro-computed tomography
  • Osteoclast
  • Pseudomonas aeruginosa


Dive into the research topics of 'Radiographic and micro-computed tomographic imaging of lipopolysaccharide- mediated bone resorption'. Together they form a unique fingerprint.

Cite this