TY - JOUR
T1 - Quercetin inhibits peripheral and spinal cord nociceptive mechanisms to reduce intense acute swimming-induced muscle pain in mice
AU - Borghi, Sergio M.
AU - Pinho-Ribeiro, Felipe A.
AU - Fattori, Victor
AU - Bussmann, Allan J.C.
AU - Vignoli, Josiane A.
AU - Camilios-Neto, Doumit
AU - Casagrande, Rubia
AU - Verri, Waldiceu A.
N1 - Funding Information:
This work received financial support from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação Araucária, Paraná State Government and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). It is noteworthy to mention that this manuscript has been read and approved by all the authors, which contributed substantially to the work. The authors declare no conflict of interest.
Publisher Copyright:
© 2016 Borghi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2016/9
Y1 - 2016/9
N2 - The present study aimed to evaluate the effects of the flavonoid quercetin (3,3',4',5,7-pentahydroxyflavone) in a mice model of intense acute swimming-induced muscle pain, which resembles delayed onset muscle soreness. Quercetin intraperitoneal (i.p.) treatment dose-dependently reduced muscle mechanical hyperalgesia. Quercetin inhibited myeloperoxidase (MPO) and N-acetyl-ß-D- glucosaminidase (NAG) activities, cytokine production, oxidative stress, cyclooxygenase-2 (COX-2) and gp91phox mRNA expression and muscle injury (creatinine kinase [CK] blood levels and myoblast determination protein [MyoD] mRNA expression) as well as inhibited NF?B activation and induced Nrf2 and HO- 1 mRNA expression in the soleus muscle. Beyond inhibiting those peripheral effects, quercetin also inhibited spinal cord cytokine production, oxidative stress and glial cells activation (glial fibrillary acidic protein [GFAP] and ionized calcium-binding adapter molecule 1 [Iba-1] mRNA expression). Concluding, the present data demonstrate that quercetin is a potential molecule for the treatment of muscle pain conditions related to unaccustomed exercise.
AB - The present study aimed to evaluate the effects of the flavonoid quercetin (3,3',4',5,7-pentahydroxyflavone) in a mice model of intense acute swimming-induced muscle pain, which resembles delayed onset muscle soreness. Quercetin intraperitoneal (i.p.) treatment dose-dependently reduced muscle mechanical hyperalgesia. Quercetin inhibited myeloperoxidase (MPO) and N-acetyl-ß-D- glucosaminidase (NAG) activities, cytokine production, oxidative stress, cyclooxygenase-2 (COX-2) and gp91phox mRNA expression and muscle injury (creatinine kinase [CK] blood levels and myoblast determination protein [MyoD] mRNA expression) as well as inhibited NF?B activation and induced Nrf2 and HO- 1 mRNA expression in the soleus muscle. Beyond inhibiting those peripheral effects, quercetin also inhibited spinal cord cytokine production, oxidative stress and glial cells activation (glial fibrillary acidic protein [GFAP] and ionized calcium-binding adapter molecule 1 [Iba-1] mRNA expression). Concluding, the present data demonstrate that quercetin is a potential molecule for the treatment of muscle pain conditions related to unaccustomed exercise.
UR - http://www.scopus.com/inward/record.url?scp=84991010117&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0162267
DO - 10.1371/journal.pone.0162267
M3 - Article
C2 - 27583449
AN - SCOPUS:84991010117
SN - 1932-6203
VL - 11
JO - PloS one
JF - PloS one
IS - 9
M1 - e0162267
ER -