TY - JOUR
T1 - Quercetin attenuates zymosan-induced arthritis in mice
AU - Guazelli, Carla F.S.
AU - Staurengo-Ferrari, Larissa
AU - Zarpelon, Ana C.
AU - Pinho-Ribeiro, Felipe A.
AU - Ruiz-Miyazawa, Kenji W.
AU - Vicentini, Fabiana T.M.C.
AU - Vignoli, Josiane A.
AU - Camilios-Neto, Doumit
AU - Georgetti, Sandra R.
AU - Baracat, Marcela M.
AU - Casagrande, Rubia
AU - Verri, Waldiceu A.
N1 - Funding Information:
This work was supported by grants from CNPq , CAPES , Fundação Araucária and Governo do Estado do Paraná (Brazil) . LSF receive postdoctoral fellowship from CAPES. RC and WAVJ receive senior research fellowship from CNPq.
Publisher Copyright:
© 2018 Elsevier Masson SAS
PY - 2018/6
Y1 - 2018/6
N2 - Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by articular lesions, recruitment of inflammatory cells and increased levels of pro-inflammatory cytokine. The intra-articular administration of zymosan is an experimental model that promotes inflammatory parameters resembling RA. Therefore, this model was used to investigate the efficacy of quercetin as a treatment of articular inflammation. Treatment with quercetin dose-dependently reduced zymosan-induced hyperalgesia, articular edema and the recruitment of neutrophils to the knee joint cavity. Histological analysis confirmed that quercetin inhibited zymosan-induced arthritis. The treatment with quercetin also inhibited zymosan-induced depletion of reduced glutathione (GSH) levels, TNFα and IL-1β production, and gp91phox, prepro-endothelin-1 (preproET-1), and cyclooxygenase-2 mRNA expression. These molecular effects of quercetin were related to the inhibition of the nuclear factor kappa-B and induction of Nuclear factor erythroid 2- related factor (Nrf2)/home oxygenase (HO-1) pathway. Thus, quercetin exerted anti-inflammatory, analgesic and antioxidant effects in experimental arthritis, suggesting quercetin is a possible candidate for arthritis treatment.
AB - Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by articular lesions, recruitment of inflammatory cells and increased levels of pro-inflammatory cytokine. The intra-articular administration of zymosan is an experimental model that promotes inflammatory parameters resembling RA. Therefore, this model was used to investigate the efficacy of quercetin as a treatment of articular inflammation. Treatment with quercetin dose-dependently reduced zymosan-induced hyperalgesia, articular edema and the recruitment of neutrophils to the knee joint cavity. Histological analysis confirmed that quercetin inhibited zymosan-induced arthritis. The treatment with quercetin also inhibited zymosan-induced depletion of reduced glutathione (GSH) levels, TNFα and IL-1β production, and gp91phox, prepro-endothelin-1 (preproET-1), and cyclooxygenase-2 mRNA expression. These molecular effects of quercetin were related to the inhibition of the nuclear factor kappa-B and induction of Nuclear factor erythroid 2- related factor (Nrf2)/home oxygenase (HO-1) pathway. Thus, quercetin exerted anti-inflammatory, analgesic and antioxidant effects in experimental arthritis, suggesting quercetin is a possible candidate for arthritis treatment.
KW - Inflammation
KW - NF-κB
KW - Nrf2/heme Oxygenase (HO-1) pathway
KW - Oxidative stress
KW - Quercetin
KW - Rheumatoid arthritis
UR - http://www.scopus.com/inward/record.url?scp=85044000031&partnerID=8YFLogxK
U2 - 10.1016/j.biopha.2018.03.057
DO - 10.1016/j.biopha.2018.03.057
M3 - Article
C2 - 29554596
AN - SCOPUS:85044000031
SN - 0753-3322
VL - 102
SP - 175
EP - 184
JO - Biomedicine and Pharmacotherapy
JF - Biomedicine and Pharmacotherapy
ER -