Quantum dot labelling of adenovirus allows highly sensitive single cell flow and imaging cytometry

Morgan R. Herod; Robert G. Pineda; Vivien Mautner; David Onion

doi:10.1002/smll.201401885

Quantum dot labelling of adenovirus allows highly sensitive single cell flow and imaging cytometry

Morgan R. Herod
, Robert G. Pineda
, Vivien Mautner
, David Onion

Program in Occupational Therapy

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of techniques including imaging cytometry and confocal microscopy. The method is applied to characterise the tropism of different adenoviral vectors.

Original language	English
Pages (from-to)	797-803
Number of pages	7
Journal	Small
Volume	11
Issue number	7
DOIs	https://doi.org/10.1002/smll.201401885
State	Published - Feb 18 2015

Keywords

Adenovirus
Flow cytometry
Gene therapy
Imaging cytometry
Quantum dots

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10.1002/smll.201401885

Cite this

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abstract = "A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of techniques including imaging cytometry and confocal microscopy. The method is applied to characterise the tropism of different adenoviral vectors.",

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AU - Pineda, Robert G.

AU - Mautner, Vivien

AU - Onion, David

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AB - A quantum dot method for highly efficient labelling of single adenoviral particles is developed. The technique has no impact on viral fitness and allows the imaging and tracking of virus binding and internalisation events using a variety of techniques including imaging cytometry and confocal microscopy. The method is applied to characterise the tropism of different adenoviral vectors.

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KW - Flow cytometry

KW - Gene therapy

KW - Imaging cytometry

KW - Quantum dots

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Quantum dot labelling of adenovirus allows highly sensitive single cell flow and imaging cytometry

Abstract

Keywords

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