TY - JOUR
T1 - Quantitative Visualization of Nanoscale Ion Transport
AU - Zhou, Lushan
AU - Gong, Yongfeng
AU - Hou, Jianghui
AU - Baker, Lane A.
N1 - Funding Information:
This work was supported by National Institute of Diabetes and Digestive and Kidney Diseases Grant R01DK084059, American Heart Association Grant 17SDG33410806, and Department of Defense Grant HDTRA11510032. We thank David Bancroft for the great help with the modification of FPGA of the customized instrument.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/12/19
Y1 - 2017/12/19
N2 - Understanding ion transport properties at various interfaces, especially at small length scales, is critical in advancing our knowledge of membrane materials and cell biology. Recently, we described potentiometric-scanning ion conductance microscopy (P-SICM) for ion-conductance measurement in polymer membranes and epithelial cell monolayers at discrete points in a sample. Here, we combine hopping mode techniques with P-SICM to allow simultaneous nanometer-scale conductance and topography mapping. First validated with standard synthetic membranes and then demonstrated in living epithelial cell monolayers under physiological conditions, this new method allows direct visualization of heterogeneous ion transport of biological samples for the first time. These advances provide a noncontact local probe, require no labeling, and present a new tool for quantifying intrinsic transport properties of a variety of biological samples.
AB - Understanding ion transport properties at various interfaces, especially at small length scales, is critical in advancing our knowledge of membrane materials and cell biology. Recently, we described potentiometric-scanning ion conductance microscopy (P-SICM) for ion-conductance measurement in polymer membranes and epithelial cell monolayers at discrete points in a sample. Here, we combine hopping mode techniques with P-SICM to allow simultaneous nanometer-scale conductance and topography mapping. First validated with standard synthetic membranes and then demonstrated in living epithelial cell monolayers under physiological conditions, this new method allows direct visualization of heterogeneous ion transport of biological samples for the first time. These advances provide a noncontact local probe, require no labeling, and present a new tool for quantifying intrinsic transport properties of a variety of biological samples.
UR - http://www.scopus.com/inward/record.url?scp=85038860489&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.7b04139
DO - 10.1021/acs.analchem.7b04139
M3 - Article
C2 - 29164870
AN - SCOPUS:85038860489
VL - 89
SP - 13603
EP - 13609
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 24
ER -