Quantitative phase microscopy by a glass microsphere and plate

Vahid Abbasian, Arash Darafsheh

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


This paper introduces a simple arrangement for quantitative phase microscopy (QPM). We implement a compact and low-cost self-referencing setup for digital holographic microscopy (DHM). A glass plate (GP) is used to implement lateral shearing interferometry, and a glass microsphere (MS) takes on the responsibility of an objective lens. The usefulness and capability of the proposed system are validated by using it to resolve a standard grating structure, followed by validation with the results obtained from atomic force microscopy (AFM). We applied the setup to perform 3D visualizations of human red blood cells, demonstrating the technique's potential for high-contrast label-free QPM of biological samples and its utility in cell morphology, identification, and classification.

Original languageEnglish
Title of host publicationQuantitative Phase Imaging X
EditorsYang Liu, YongKeun Park
ISBN (Electronic)9781510669635
StatePublished - 2024
EventQuantitative Phase Imaging X 2024 - San Francisco, United States
Duration: Jan 27 2024Jan 29 2024

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceQuantitative Phase Imaging X 2024
Country/TerritoryUnited States
CitySan Francisco


  • Digital holographic microscopy
  • Microsphere-assisted microscopy,3D imaging
  • Quantitative phase microscopy


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