TY - GEN
T1 - Quantitative phase microscopy by a glass microsphere and plate
AU - Abbasian, Vahid
AU - Darafsheh, Arash
N1 - Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.
PY - 2024
Y1 - 2024
N2 - This paper introduces a simple arrangement for quantitative phase microscopy (QPM). We implement a compact and low-cost self-referencing setup for digital holographic microscopy (DHM). A glass plate (GP) is used to implement lateral shearing interferometry, and a glass microsphere (MS) takes on the responsibility of an objective lens. The usefulness and capability of the proposed system are validated by using it to resolve a standard grating structure, followed by validation with the results obtained from atomic force microscopy (AFM). We applied the setup to perform 3D visualizations of human red blood cells, demonstrating the technique's potential for high-contrast label-free QPM of biological samples and its utility in cell morphology, identification, and classification.
AB - This paper introduces a simple arrangement for quantitative phase microscopy (QPM). We implement a compact and low-cost self-referencing setup for digital holographic microscopy (DHM). A glass plate (GP) is used to implement lateral shearing interferometry, and a glass microsphere (MS) takes on the responsibility of an objective lens. The usefulness and capability of the proposed system are validated by using it to resolve a standard grating structure, followed by validation with the results obtained from atomic force microscopy (AFM). We applied the setup to perform 3D visualizations of human red blood cells, demonstrating the technique's potential for high-contrast label-free QPM of biological samples and its utility in cell morphology, identification, and classification.
KW - Digital holographic microscopy
KW - Microsphere-assisted microscopy,3D imaging
KW - Quantitative phase microscopy
UR - http://www.scopus.com/inward/record.url?scp=85191016008&partnerID=8YFLogxK
U2 - 10.1117/12.3000264
DO - 10.1117/12.3000264
M3 - Conference contribution
AN - SCOPUS:85191016008
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Quantitative Phase Imaging X
A2 - Liu, Yang
A2 - Park, YongKeun
PB - SPIE
T2 - Quantitative Phase Imaging X 2024
Y2 - 27 January 2024 through 29 January 2024
ER -