Quantitative diffuse optical tomography for small animals using an ultrafast gated image intensifier

Sachin V. Patwardhan, Joseph P. Culver

Research output: Contribution to journalArticlepeer-review

33 Scopus citations


The quantitative accuracy of fluorescence and bioluminescence imaging of small animals can be improved by knowledge of the in situ optical properties of each animal. Obtaining in situ optical property maps is challenging, however, due to short propagation distances, requirements for high dynamic range, and the need for dense spatial, temporal, and spectral sampling. Using an ultrafast gated image intensifier and a pulsed laser source, we have developed a small animal diffuse optical tomography system with multiple synthetic modulation frequencies up to >1GHz. We show that amplitude and phase measurements with useful contrast-to-noise ratios can be obtained for modulation frequencies over the range of ∼250 to 1250MHz. Experiments with tissue simulating phantoms demonstrate the feasibility of reconstructing the absorption and scattering optical properties in a small animal imaging system.

Original languageEnglish
Article number011009
JournalJournal of biomedical optics
Issue number1
StatePublished - 2008


  • diffuse optical tomography
  • fluorescence imaging
  • molecular imaging
  • near-infrared (NIR) spectroscopy
  • small animal imaging


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