Quantitative differential interference contrast microscopy based on structured-aperture interference

Xiquan Cui; Matthew Lew; Changhuei Yang

doi:10.1063/1.2977870

Quantitative differential interference contrast microscopy based on structured-aperture interference

Xiquan Cui, Matthew Lew, Changhuei Yang

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Abstract

We report a quantitative differential interference contrast (DIC) microscope based on a structured-aperture (SA) wavefront sensor. Unlike a conventional DIC microscope, the SA-DIC microscope can separate the amplitude and the phase gradient information of the image wavefront, and form quantitative intensity and DIC images of the sample with good resolution; our prototype achieved resolution ∼2 μm. Furthermore, due to the nonpolarization nature of the microscope, we were able to image birefringent samples without artifacts.

Original language	English
Article number	091113
Journal	Applied Physics Letters
Volume	93
Issue number	9
DOIs	https://doi.org/10.1063/1.2977870
State	Published - 2008

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title = "Quantitative differential interference contrast microscopy based on structured-aperture interference",

abstract = "We report a quantitative differential interference contrast (DIC) microscope based on a structured-aperture (SA) wavefront sensor. Unlike a conventional DIC microscope, the SA-DIC microscope can separate the amplitude and the phase gradient information of the image wavefront, and form quantitative intensity and DIC images of the sample with good resolution; our prototype achieved resolution ∼2 μm. Furthermore, due to the nonpolarization nature of the microscope, we were able to image birefringent samples without artifacts.",

author = "Xiquan Cui and Matthew Lew and Changhuei Yang",

note = "Funding Information: The authors acknowledge financial support from the Defense Advanced Research Projects Agency Center for Optofluidic Integration. We thank Professor Paul Sternberg, Dr. Ying Zhou, Jigang Wu, Guoan Zheng, and Emily McDowell for their technical assistance and enlightening discussions.",

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AU - Lew, Matthew

AU - Yang, Changhuei

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PY - 2008

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N2 - We report a quantitative differential interference contrast (DIC) microscope based on a structured-aperture (SA) wavefront sensor. Unlike a conventional DIC microscope, the SA-DIC microscope can separate the amplitude and the phase gradient information of the image wavefront, and form quantitative intensity and DIC images of the sample with good resolution; our prototype achieved resolution ∼2 μm. Furthermore, due to the nonpolarization nature of the microscope, we were able to image birefringent samples without artifacts.

AB - We report a quantitative differential interference contrast (DIC) microscope based on a structured-aperture (SA) wavefront sensor. Unlike a conventional DIC microscope, the SA-DIC microscope can separate the amplitude and the phase gradient information of the image wavefront, and form quantitative intensity and DIC images of the sample with good resolution; our prototype achieved resolution ∼2 μm. Furthermore, due to the nonpolarization nature of the microscope, we were able to image birefringent samples without artifacts.

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Quantitative differential interference contrast microscopy based on structured-aperture interference

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