Quantitative cytochemistry of the organ of corti. Dissection, weight determination and analysis of single outer hair cells

R. Thalmann, I. Thalmann, T. H. Comegys

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

A method is described for the dissection of single outer hair cells in the freeze-dried state. The average dry weight of outer hair cells from the third and fourth cochlear turn in the guinea pig is of the order of one nanogram, with variations depending upon the precise location. The measured dry weight is in line with the wet weight estimated from the volume of living outer hair cells maintained under iso-osmotic conditions. The lipid fraction of outer hair cells amounts to about 21 percent of the dry weight. Average ATP levels in outer hair cells of the upper two turns range between 12-15 mmoles/kg dry weight, with no significant difference between rows or turns.

Original languageEnglish
Pages (from-to)2059-2078
Number of pages20
JournalLaryngoscope
Volume82
Issue number11
DOIs
StatePublished - Nov 1972

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