@inbook{aac59f7879354324a48374f3c2f2224d,
title = "Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation",
abstract = "Transcription factors control gene expression by binding to noncoding regions of DNA known as {\^A} cis-regulatory elements (CREs i.e., enhancer/promoters). Traditionally, cis-regulatory analysis has been carried out via mouse transgenesis which is time1-consuming and nonquantitative. Electroporation of DNA reporter constructs into living mouse tissue is a rapid and effective alternative to transgenesis which permits quantitative assessment of cis-regulatory activity. Here, we present a simple technique for quantifying the activity of photoreceptor-specific CREs in living explanted mouse retinas.",
keywords = "Electroporation, Mouse, Photoreceptor, Quantification, Retina, cis-Regulatory element",
author = "Montana, {Cynthia L.} and Myers, {Connie A.} and Corbo, {Joseph C.}",
year = "2013",
doi = "10.1007/978-1-62703-080-9-23",
language = "English",
isbn = "9781627030793",
series = "Methods in Molecular Biology",
pages = "329--340",
editor = "Weber, {Bernhard H.F.} and Thomas Langmann",
booktitle = "Retinal Degeneration",
}