Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation

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4 Scopus citations

Abstract

Transcription factors control gene expression by binding to noncoding regions of DNA known as  cis-regulatory elements (CREs i.e., enhancer/promoters). Traditionally, cis-regulatory analysis has been carried out via mouse transgenesis which is time1-consuming and nonquantitative. Electroporation of DNA reporter constructs into living mouse tissue is a rapid and effective alternative to transgenesis which permits quantitative assessment of cis-regulatory activity. Here, we present a simple technique for quantifying the activity of photoreceptor-specific CREs in living explanted mouse retinas.

Original languageEnglish
Title of host publicationRetinal Degeneration
Subtitle of host publicationMethods and Protocols
EditorsBernhard H.F. Weber, Thomas Langmann
Pages329-340
Number of pages12
DOIs
StatePublished - Jan 10 2013

Publication series

NameMethods in Molecular Biology
Volume935
ISSN (Print)1064-3745

Keywords

  • Electroporation
  • Mouse
  • Photoreceptor
  • Quantification
  • Retina
  • cis-Regulatory element

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