TY - JOUR
T1 - Quantifying storage material accumulation in tissue sections
AU - Cooper, Jonathan D.
AU - Brooks, Helen R.E.
AU - Nelvagal, Hemanth R.
N1 - Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015
Y1 - 2015
N2 - The ability to reliably quantify the relative degree of storage burden that results from lysosomal dysfunction is an important goal. Such measurements not only allow an assessment of different stages of disease progression, but also the assessment of therapeutic strategies. Although biochemical methods exist for doing this, retaining the anatomical integrity of tissue samples is an important consideration. This chapter provides practical methodological recommendations for achieving this goal in tissue sections, either by directly visualizing or staining the storage material, and subsequently quantifying it via image analysis.
AB - The ability to reliably quantify the relative degree of storage burden that results from lysosomal dysfunction is an important goal. Such measurements not only allow an assessment of different stages of disease progression, but also the assessment of therapeutic strategies. Although biochemical methods exist for doing this, retaining the anatomical integrity of tissue samples is an important consideration. This chapter provides practical methodological recommendations for achieving this goal in tissue sections, either by directly visualizing or staining the storage material, and subsequently quantifying it via image analysis.
KW - Assessing therapeutic efficacy
KW - Autofluorescence
KW - Confocal microscopy
KW - Disease progression
KW - Immunostaining
KW - Lysosomal dysfunction
KW - Quantitative analysis
KW - Storage material accumulation
KW - Thresholding image analysis
UR - http://www.scopus.com/inward/record.url?scp=84922858395&partnerID=8YFLogxK
U2 - 10.1016/bs.mcb.2014.11.010
DO - 10.1016/bs.mcb.2014.11.010
M3 - Article
C2 - 25665454
AN - SCOPUS:84922858395
SN - 0091-679X
VL - 126
SP - 349
EP - 356
JO - Methods in cell biology
JF - Methods in cell biology
ER -