Purpose. To assess the feasibility of our newly developed cardiovascular magnetic resonance (CMR) methods to quantify global and/or regional myocardial oxygen consumption rate (MVO2) at rest and during pharmacologically-induced vasodilation in normal volunteers. Methods. A breath-hold T2quantification method is developed to calculate oxygen extraction fraction (OEF) and MVO2rate at rest and/or during hyperemia, using a two-compartment model. A previously reported T 2quantification method using turbo-spin-echo sequence was also applied for comparison. CMR scans were performed in 6 normal volunteers. Each imaging session consisted of imaging at rest and during adenosine-induced vasodilation. The new T2quantification method was applied to calculate T2in the coronary sinus (CS), as well as in myocardial tissue. Resting CS OEF, representing resting global myocardial OEF, and myocardial OEF during adenosine vasodilation were then calculated by the model. Myocardial blood flow (MBF) was also obtained to calculate MVO2, by using a first-pass perfusion imaging approach. Results. The T 2quantification method yielded a hyperemic OEF of 0.37 0.05 and a hyperemic MVO2of 9.2 2.4 mol/g/min. The corresponding resting values were 0.73 0.05 and 5.2 1.7 mol/g/min respectively, which agreed well with published literature values. The MVO2rose proportionally with rate-pressure product from the rest condition. The T2sensitivity is approximately 95% higher with the new T2method than turbo-spin-echo method. Conclusion. The CMR oxygenation method demonstrates the potential for non-invasive estimation of myocardial oxygenation, and should be explored in patients with altered myocardial oxygenation.