Quantification of F2-isoprostanes as a biomarker of oxidative stress

Ginger L. Milne, Stephanie C. Sanchez, Erik S. Musiek, Jason D. Morrow

Research output: Contribution to journalArticle

225 Scopus citations

Abstract

Oxidant stress has been implicated in a wide variety of disease processes. One method to quantify oxidative injury is to measure lipid peroxidation. Quantification of a group of prostaglandin F-like compounds derived from the nonezymatic oxidation of arachidonic acid, termed the F2-isoprostanes (F2-IsoPs), provides an accurate assessment of oxidative stress both in vitro and in vivo. In fact, in a recent independent study sponsored by the National Institutes of Health (NIH), F2-IsoPs were shown to be the most reliable index of in vivo oxidant stress when compared against other well known biomarkers. This protocol details our laboratory's method to quantify F2-IsoPs in biological fluids and tissues using gas chromatography-mass spectrometry (GC-MS). This procedure can be completed for 12-15 samples in 6-8 h.

Original languageEnglish
Pages (from-to)221-226
Number of pages6
JournalNature Protocols
Volume2
Issue number1
DOIs
StatePublished - Feb 1 2007
Externally publishedYes

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