Abstract
Quantification of changes in the total length of randomly oriented and possibly curved lines appearing in an image is a necessity in a wide variety of biological applications. Here, we present an automated approach based upon Fourier space analysis. Scaled, band-pass filtered power spectral densities of greyscale images are integrated to provide a quantitative measurement of the total length of lines of a particular range of thicknesses appearing in an image. A procedure is presented to correct for changes in image intensity. The method is most accurate for two-dimensional processes with fibres that do not occlude one another.
| Original language | English |
|---|---|
| Pages (from-to) | 2310-2329 |
| Number of pages | 20 |
| Journal | Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences |
| Volume | 467 |
| Issue number | 2132 |
| DOIs | |
| State | Published - Aug 8 2011 |
Keywords
- Automated image processing
- Cellular mechanics
- Fibre length measurement