Abstract

Quantification of changes in the total length of randomly oriented and possibly curved lines appearing in an image is a necessity in a wide variety of biological applications. Here, we present an automated approach based upon Fourier space analysis. Scaled, band-pass filtered power spectral densities of greyscale images are integrated to provide a quantitative measurement of the total length of lines of a particular range of thicknesses appearing in an image. A procedure is presented to correct for changes in image intensity. The method is most accurate for two-dimensional processes with fibres that do not occlude one another.

Original languageEnglish
Pages (from-to)2310-2329
Number of pages20
JournalProceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences
Volume467
Issue number2132
DOIs
StatePublished - Aug 8 2011

Keywords

  • Automated image processing
  • Cellular mechanics
  • Fibre length measurement

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