TY - JOUR
T1 - Quantification of fibre polymerization through Fourier space image analysis
AU - Nekouzadeh, Ali
AU - Genin, Guy M.
PY - 2011/8/8
Y1 - 2011/8/8
N2 - Quantification of changes in the total length of randomly oriented and possibly curved lines appearing in an image is a necessity in a wide variety of biological applications. Here, we present an automated approach based upon Fourier space analysis. Scaled, band-pass filtered power spectral densities of greyscale images are integrated to provide a quantitative measurement of the total length of lines of a particular range of thicknesses appearing in an image. A procedure is presented to correct for changes in image intensity. The method is most accurate for two-dimensional processes with fibres that do not occlude one another.
AB - Quantification of changes in the total length of randomly oriented and possibly curved lines appearing in an image is a necessity in a wide variety of biological applications. Here, we present an automated approach based upon Fourier space analysis. Scaled, band-pass filtered power spectral densities of greyscale images are integrated to provide a quantitative measurement of the total length of lines of a particular range of thicknesses appearing in an image. A procedure is presented to correct for changes in image intensity. The method is most accurate for two-dimensional processes with fibres that do not occlude one another.
KW - Automated image processing
KW - Cellular mechanics
KW - Fibre length measurement
UR - http://www.scopus.com/inward/record.url?scp=80051734379&partnerID=8YFLogxK
U2 - 10.1098/rspa.2010.0623
DO - 10.1098/rspa.2010.0623
M3 - Article
AN - SCOPUS:80051734379
SN - 1364-5021
VL - 467
SP - 2310
EP - 2329
JO - Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences
JF - Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences
IS - 2132
ER -